CD54 (ICAM-1), clone: HA58, eBioscience™
Mouse Monoclonal Antibody
Brand: Affymetrix eBioscience 14-0549-82
Code : Z2
Additional Details : Weight : 0.01000kg
DescriptionDescription: The HA58 monoclonal antibody reacts with human CD54 (InterCellular Adhesion Molecule-1, ICAM-1), a 90-110kDa transmembrane glycoprotein expressed by monocytes, lymphocytes and endothelial cells. Expression of CD54 is upregulated on activated lymphocytes. Interaction of CD54 with its ligand CD11a is important in the inflammatory response.Applications Reported: This HA58 antibody has been reported for use in flow cytometric analysis, immunoblotting (WB), and immunohistochemical staining. HA58 has also been reported in blocking of in vitro mixed lymphocyte reaction. (Please use Functional Grade purified HA58, cat. 16-0549, in functional assays.).Applications Tested: The HA58 antibody has been tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at less than or equal to 0.25 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Purity: Greater than 90%, as determined by SDS-PAGE.Aggregation: Less than 10%, as determined by HPLC.Filtration: 0.2μm post-manufacturing filtered.
|PBS with 0.09% sodium azide; pH 7.2|
|BB2, P3.58, ICAM-1, cell surface glycoprotein P3.58, intercellular adhesion molecule 1, human rhinovirus receptor, major group rhinovirus receptor|
|Flow CytometryþFunctional AssayþImmunohistochemistryþWestern Blot|
|Store at 2-8°C.|
For Research Use Only.