CD5 Monoclonal Antibody (YKIX322.3), PerCP-eFluor™ 710, eBioscience™, Invitrogen™

Rat Monoclonal Antibody

Product Code	Brand	Quantity
15589256	Affymetrix eBioscience 46-5050-42	100 Tests

Description

Description: This YKIX322.3 monoclonal antibody reacts with canine CD5, also known as Ly-1. CD5 is a 67-kDa type I transmembrane glycoprotein expressed on thymocytes and mature T cells. Studies have demonstrated that NK cells in dogs also express CD5, but at lower levels. In dogs, CD5 is not detectable on B cells or B cell lines. Applications Reported: This YKIX322.3 antibody has been reported for use in flow cytometric analysis. Applications Tested: This YKIX322.3 antibody has been pre-titrated and tested by flow cytometric analysis of normal canine peripheral blood cells. This can be used at 5 μL (0.125 μg) per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. PerCP-eFluor™ 710 can be used in place of PE-Cy5, PE-Cy5.5 or PerCP-Cy5.5. PerCP-eFluor™ 710 emits at 710 nm and is excited with the blue laser (488 nm). Please make sure that your instrument is capable of detecting this fluorochrome. For a filter configuration, we recommend using the 685 LP dichroic mirror and 710/40 band pass filter, however the 695/40 band pass filter is an acceptable alternative.

CD5 Monoclonal antibody specifically detects CD5 in Canine samples. It is validated for Flow Cytometry.

Specifications

• Antigen: CD5
• Concentration: 5 μL/Test
• Classification: Monoclonal
• Form: Liquid
• Regulatory Status: RUO
• Isotype: IgG2a κ
• Purification Method: Affinity chromatography
• Product Type: Antibody

• Clone: YKIX322.3
• Applications: Flow Cytometry
• Conjugate: PerCP-eFluor 710
• Host Species: Rat
• Target Species: Canine
• Primary or Secondary: Primary
• Content And Storage: 4° C, store in dark, DO NOT FREEZE!

For Research Use Only.