CD5, PerCP-eFluor™ 710, clone: YKIX322.3, eBioscience™

Rat Monoclonal Antibody

Brand:  Affymetrix eBioscience 46-5050-42

Product Code. 15589256

Description

Description: This YKIX322.3 monoclonal antibody reacts with canine CD5, also known as Ly-1. CD5 is a 67-kDa type I transmembrane glycoprotein expressed on thymocytes and mature T cells. Studies have demonstrated that NK cells in dogs also express CD5, but at lower levels. In dogs, CD5 is not detectable on B cells or B cell lines.Applications Reported: This YKIX322.3 antibody has been reported for use in flow cytometric analysis.Applications Tested: This YKIX322.3 antibody has been pre-titrated and tested by flow cytometric analysis of normal canine peripheral blood cells. This can be used at 5 μL (0.125 μg) per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. PerCP-eFluor® 710 can be used in place of PE-Cy5, PE-Cy5.5 or PerCP-Cy5.5. PerCP-eFluor® 710 emits at 710 nm and is excited with the blue laser (488 nm). Please make sure that your instrument is capable of detecting this fluorochrome. For a filter configuration, we recommend using the 685 LP dichroic mirror and 710/40 band pass filter, however the 695/40 band pass filter is an acceptable alternative. Our testing indicates that PerCP-eFluor® 710 conjugated antibodies are stable when stained samples are exposed to freshly prepared 2% formaldehyde overnight at 4°C, but please evaluate for alternative fixation protocols.Excitation: 488 nm; Emission: 710 nm; Laser: Blue Laser.Filtration: 0.2μm post-manufacturing filtered.

Specifications

• Antigen: CD5
• Classification: Monoclonal
• Concentration: 5μl/Test
• Formulation: PBS with 0.1% gelatin, 0.2% BSA and 0.09% sodium azide; pH 7.2
• Format: Conjugated
• Gene Symbols: CD28
• Isotype: IgG2a, kappa
• Quantity: 100 Tests
• Storage Requirements: Store at 2-8°C. Do not freeze. Light-sensitive material.
• Primary or Secondary: Primary
• Target Species: Canine

• Applications: Flow Cytometry
• Clone: YKIX322.3
• Conjugate: PerCP-eFluor 710
• Gene: CD28
• Gene Alias: CD5 antigen p56-62, T-cell surface glycoprotein CD5, lymphocyte antigen T1/Leu-1
• Host Species: Rat
• Purification Method: Affinity chromatography
• Regulatory Status: RUO
• Type: Antibody
• Monoclonal or Polyclonal: Monoclonal

For Research Use Only.