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CD5, PE-Cyanine5, clone: L17F12, eBioscience™

Mouse Monoclonal Antibody

Brand:  Affymetrix eBioscience 15-0058-42

Code : NEW

Additional Details : Weight : 0.23750kg

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Product Code. 15567706

  • £214.45 / 100 tests


Estimated Shipment date
from Supplier 05-06-2018
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Description

Description

Description: The L17F12 monoclonal antibody reacts with human CD5, a 67kDa protein expressed by a majority of thymocytes and mature T cells and a subset of B cells. Signaling through the CD5 molecule activates T cells and binding of CD5 to its ligand on B cells, CD72, and plays an important role in T-B interaction and proliferation.Applications Reported: This L17F12 antibody has been reported for use in flow cytometric analysis.Applications Tested: This L17F12 antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at 5 μL (0.06 μg) per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. Light sensitivity: This tandem dye is sensitive to photo-induced oxidation. Please protect this vial and stained samples from light.Fixation: Samples can be stored in IC Fixation Buffer (cat. 00-8222) (100 μL of cell sample + 100 μL of IC Fixation Buffer) or 1-step Fix/Lyse Solution (cat. 00-5333) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically.Excitation: 488-561 nm; Emission: 667 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.Filtration: 0.2μm post-manufacturing filtered.
Specifications

Specifications

CD5
Monoclonal
5μl/Test
PBS with 0.2% BSA and 0.09% sodium azide; pH 7.2
P06127
CD5 antigen p56-62, T-cell surface glycoprotein CD5, lymphocyte antigen T1/Leu-1
Murine
Affinity chromatography
RUO
Antibody
Monoclonal
Human
Flow Cytometry
L17F12
PE-Cyanine5
CD5
Conjugated
CD5
IgG2a, kappa
100 Tests
Store at 2-8°C. Do not freeze. Light-sensitive material.
Primary
921
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