CD49e (Integrin alpha 5), clone: eBioHMa5-1 (HMa5-1), eBioscience™
Armenian Hamster Monoclonal Antibody
Brand: Affymetrix eBioscience 14-0493-85
Code : Z2
Additional Details : Weight : 0.01000kg
DescriptionDescription: The eBioHMalpha5-1 monoclonal antibody reacts with mouse and rat CD49e, also known as integrin alpha5 or VLA-5A. CD49e is a member of the integrin alpha chain family, and binds with CD29 (integrin beta1) to form the receptor VLA-5, which binds the ligands fibronectin and fibrinogen. CD49e is a type I, single-pass membrane protein, with a molecular weight of 135 kDa, and is expressed on multiple cell types including thymocytes, mast cells, activated T cells and splenic B cells. In addition to its role in adhesion, VLA-5 contributes to T-cell co-stimulation. The eBioHMalpha5-1 monoclonal antibody has been demonstrated to interfere with VLA-5-mediated cell adhesion, and with the degranulation of IgE-sensitized rat RBL cells stimulated on fibronectin-coated plates. Furthermore, HMalpha5-1 injected into mice subcutaneously, along with anti-CD49d and anti-CD61, is able to inhibit passive cutaneous anaphylaxis.Applications Reported: This eBioHMa5-1 (HMa5-1) antibody has been reported for use in flow cytometric analysis, and immunoprecipitation. (Please use Functional Grade purified eBioHMa5-1 (HMa5-1), cat. 16-0493, in functional assays.).Applications Tested: This eBioHMa5-1 (HMa5-1) antibody has been tested by flow cytometric analysis of mouse bone marrow cells. This can be used at less than or equal to 1 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Purity: Greater than 90%, as determined by SDS-PAGE.Aggregation: Less than 10%, as determined by HPLC.Filtration: 0.2μm post-manufacturing filtered.
|CD49e (Integrin alpha 5)|
|PBS with 0.09% sodium azide; pH 7.2|
|FNRA, CD49e, VLA5A, ITGA5|
|Flow CytometryþFunctional AssayþImmunoprecipitation|
|Store at 2-8°C.|
For Research Use Only.