CD49d (Integrin alpha 4) Monoclonal Antibody (R1-2), PerCP-eFluor™ 710, eBioscience™, Invitrogen™

Rat Monoclonal Antibody

Product Code	Brand	Quantity
15381260	Affymetrix eBioscience 46-0492-82	100 μg

Description

Description: The R1-2 monoclonal antibody reacts with mouse CD49d, the 150 kDa integrin alpha4 subunit. The complex of CD49d non-covalently associated with integrin beta1 (CD29), also known as VLA-4, is a receptor for fibronectin and VCAM-1 (CD106). This complex is expressed by thymocytes, peripheral lymphocytes, monocytes and eosinophils. CD49d also associates with integrin b7 and binds to the Mucosal vascular Addressin (MAdCAM-1). Applications Reported: This R1-2 antibody has been reported for use in flow cytometric analysis. Applications Tested: This R1-2 antibody has been tested by flow cytometric analysis of mouse splenocytes. This can be used at less than or equal to 0.5 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. PerCP-eFluor™ 710 emits at 710 nm and is excited with the blue laser (488 nm); it can be used in place of PerCP-Cyanine5.5. We recommend using a 710/50 bandpass filter, however, the 695/40 bandpass filter is an acceptable alternative. Please make sure that your instrument is capable of detecting this fluorochrome. Fixation: Samples can be stored in IC Fixation Buffer (cat.

CD49d (Integrin alpha 4) Monoclonal antibody specifically detects CD49d (Integrin alpha 4) in Mouse samples. It is validated for Flow Cytometry.

Specifications

• Antigen: CD49d (Integrin alpha 4)
• Concentration: 0.2 mg/mL
• Classification: Monoclonal
• Form: Liquid
• Regulatory Status: RUO
• Gene Accession No.: Q00651
• Isotype: IgG2b κ
• Purification Method: Affinity chromatography
• Product Type: Antibody

• Clone: R1-2
• Applications: Flow Cytometry
• Conjugate: PerCP-eFluor 710
• Host Species: Rat
• Target Species: Mouse
• Gene ID (Entrez): 16401
• Primary or Secondary: Primary
• Content And Storage: 4° C, store in dark, DO NOT FREEZE!

For Research Use Only.