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CD49d (Integrin alpha 4), Functional Grade, clone: 9F10, eBioscience™

Mouse Monoclonal Antibody

Brand:  Affymetrix eBioscience 16-0499-85

Code : Z2

Additional Details : Weight : 0.01000kg

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Product Code. 15208477

  • £188.53 / 500µg


EU Stock 9
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Disclaimers:

For Research Use Only.


Description

Description

Description: The 9F10 monoclonal antibody reacts with human CD49d, the 150kDa integrin alpha4 subunit. The complex of CD49d non-covalently associated with integrin beta1 (CD29), also known as VLA-4, is a receptor for fibronectin and VCAM-1 (CD106). This complex is expressed by thymocytes, peripheral lymphocytes, monocytes and eosinophils. CD49d also associates with integrin beta7 and binds to the Mucosal Addressin Cell-Adhesion Molecule-1 (MadCAM-1).Applications Reported: The 9F10 antibody has been reported for use in flow cytometric analysis. 9F10 has also been reported in in vitro functional assays.Applications Tested: The 9F10 antibody has been tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at less than or equal to 1 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Endotoxin: Less than 0.001 ng/ug antibody as determined by the LAL assay.Storage and handling: Use in a sterile environment.Filtration: 0.2μm post-manufacturing filtered.Purity: Greater than 90%, as determined by SDS-PAGE.Endotoxin Level: Less than 0.001 ng/μg antibody, as determined by LAL assay.Aggregation: Less than 10%, as determined by HPLC.
Specifications

Specifications

CD49d
9F10
Unconjugated
Purified
Murine
500μg
Store at 2-8°C.
Monoclonal
Functional Assay
1mg/mL
aqueous buffer, no sodium azide
Integrin a4, VLA4, ITGA4
IgG1
RUO
Primary
Human
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