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CD49d (Integrin alpha 4), clone: 9F10, eBioscience™

Mouse Monoclonal Antibody

Brand:  Affymetrix eBioscience 14-0499-80

Code : Z2

Additional Details : Weight : 0.01000kg

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Product Code. 15207017

  • £31.01 / 25µg


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Description

Description

Description: The 9F10 monoclonal antibody reacts with human CD49d, the 150kDa integrin alpha4 subunit. The complex of CD49d non-covalently associated with integrin beta1 (CD29), also known as VLA-4, is a receptor for fibronectin and VCAM-1 (CD106). This complex is expressed by thymocytes, peripheral lymphocytes, monocytes and eosinophils. CD49d also associates with integrin beta7 and binds to the Mucosal Addressin Cell-Adhesion Molecule-1 (MadCAM-1).Applications Reported: The 9F10 antibody has been reported for use in flow cytometric analysis, and immunohistochemical staining. 9F10 has also been reported in in vitro functional assays. (Please use Functional Grade purified 9F10, cat. 16-0499, in functional assays.).Applications Tested: The 9F10 antibody has been tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at less than or equal to 0.25 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Purity: Greater than 90%, as determined by SDS-PAGE.Aggregation: Less than 10%, as determined by HPLC.Filtration: 0.2μm post-manufacturing filtered.
Specifications

Specifications

CD49d (Integrin alpha 4)
Monoclonal
0.5mg/mL
PBS with 0.09% sodium azide; pH 7.2
P13612
269C wild type, CD49 antigen-like family member D, VLA-4 subunit alpha, antigen CD49D, alpha-4 subunit of VLA-4 receptor, ITGA4, ITGA-IV
Murine
Affinity chromatography
RUO
Antibody
Monoclonal
Human
Immunohistochemistry
9F10
Unconjugated
ITGA4
Purified
ITGA4
IgG1, kappa
25μg
Store at 2-8°C.
Primary
3676
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