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CD47, Functional Grade, clone: miap301, eBioscience™

Rat Monoclonal Antibody

Brand:  Affymetrix eBioscience 16-0471-85

Code : Z2

Additional Details : Weight : 0.01000kg

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Product Code. 15278457

  • £226.40 / 500µg


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Disclaimers:

For Research Use Only.


Description

Description

Description: The monoclonal antibody miap301 reacts to CD47 also known as integrin-associated protein (IAP), and neurophilin. CD47 is a glycosylated five transmembrane protein with a small cytoplasmic domain. CD47 is involved in adhesion through interactions with SIRP (signal regulator protein) and is non-covalently associated with β3 integrins CD51/CD61, CD41/CD61. It is also known to be the receptor for thrombospondin. T cell expression of CD47 can mediate activation or apoptosis (in the presence of high levels of thrombospondin). Expression is found in the majority of hematopoietic cells including T and B cells, monocytes, platelets and erythrocytes (as part of the Rh complex). Expression is also found in non-hematopoietic cells.No crossreactivity is seen against human CD47.Applications Reported: This miap301 antibody has been reported for use in flow cytometric analysis and functional assays (blocking cell aggregation in vitro).Applications Tested: This miap301 antibody has been tested by flow cytometric analysis of mouse splenocytes. This can be used at less than or equal to 1 μg per million cells in a 100 μL total staining volume. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Endotoxin: Less than 0.001 ng/ug antibody as determined by the LAL assay.Storage and handling: Use in a sterile environment.Filtration: 0.2μm post-manufacturing filtered.Purity: Greater than 90%, as determined by SDS-PAGE.Endotoxin Level: Less than 0.001 ng/μg antibody, as determined by LAL assay.Aggregation: Less than 10%, as determined by HPLC.
Specifications

Specifications

CD47
miap301
Unconjugated
Purified
Rat
500μg
Store at 2-8°C.
Monoclonal
Functional Assay
1mg/mL
aqueous buffer, no sodium azide
Integrin associated protein, IAP
IgG2a
RUO
Primary
Murine
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