CD47, Functional Grade, clone: miap301, eBioscience™
Rat Monoclonal Antibody
Brand: Affymetrix eBioscience 16-0471-81
Code : Z2
Additional Details : Weight : 0.01000kg
DescriptionDescription: The monoclonal antibody miap301 reacts to CD47 also known as integrin-associated protein (IAP), and neurophilin. CD47 is a glycosylated five transmembrane protein with a small cytoplasmic domain. CD47 is involved in adhesion through interactions with SIRP (signal regulator protein) and is non-covalently associated with β3 integrins CD51/CD61, CD41/CD61. It is also known to be the receptor for thrombospondin. T cell expression of CD47 can mediate activation or apoptosis (in the presence of high levels of thrombospondin). Expression is found in the majority of hematopoietic cells including T and B cells, monocytes, platelets and erythrocytes (as part of the Rh complex). Expression is also found in non-hematopoietic cells.No crossreactivity is seen against human CD47.Applications Reported: This miap301 antibody has been reported for use in flow cytometric analysis and functional assays (blocking cell aggregation in vitro).Applications Tested: This miap301 antibody has been tested by flow cytometric analysis of mouse splenocytes. This can be used at less than or equal to 1 μg per million cells in a 100 μL total staining volume. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Endotoxin: Less than 0.001 ng/ug antibody as determined by the LAL assay.Storage and handling: Use in a sterile environment.Filtration: 0.2μm post-manufacturing filtered.Purity: Greater than 90%, as determined by SDS-PAGE.Endotoxin Level: Less than 0.001 ng/μg antibody, as determined by LAL assay.Aggregation: Less than 10%, as determined by HPLC.
|PBS with no preservative; pH 7.2|
|IAP, MER6, OA3, CD47 antigen, CD47 glycoprotein, Rh-related antigen, leukocyte surface antigen CD47|
|Flow CytometryþFunctional Assay|
|Store at 2-8°C.|
For Research Use Only.