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CD45RO, PE-Cyanine5, clone: UCHL1, eBioscience™

Mouse Monoclonal Antibody

Brand:  Affymetrix eBioscience 15-0457-42

Code : NEW

Additional Details : Weight : 0.23750kg

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Product Code. 15597726

  • £158.36 / 100 tests


EU Stock 1
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Description

Description

Description: The UCHL1 monoclonal antibody reacts with human CD45RO, a 180kDa isoform of CD45. CD45RO is expressed by most thymocytes, activated memory T cells, granulocytes and monocytes. CD22 is a ligand for CD45RO. Expression of CD45RO and CD45RA is used commonly to discriminate subsets of peripheral T cells.Applications Reported: The UCHL1 antibody has been reported for use in flow cytometric analysis.Applications Tested: This UCHL1 antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at 5 μL (0.03 μg) per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. Light sensitivity: This tandem dye is sensitive photo-induced oxidation. Please protect this vial and stained samples from light. Fixation: Samples can be stored in IC Fixation Buffer (cat. 00-8222) (100 μL cell sample + 100 μL IC Fixation Buffer) or 1-step Fix/Lyse Solution (cat. 00-5333) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically.Excitation: 488-561 nm; Emission: 667 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.Filtration: 0.2μm post-manufacturing filtered.
Specifications

Specifications

CD45RO
Monoclonal
5μl/Test
PBS with 0.1% gelatin, 0.2% BSA and 0.09% sodium azide; pH 7.2
P08575
B220, CD45, CD45R, GP180, LCA, LY5, T200
Murine
Affinity chromatography
RUO
Antibody
Monoclonal
Human
Flow Cytometry
UCHL1
PE-Cyanine5
PTPRC
Conjugated
PTPRC
IgG2a, kappa
100 Tests
Store at 2-8°C. Do not freeze. Light-sensitive material.
Primary
5788
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