missing translation for 'onlineSavingsMsg'
Learn More

CD45R (B220), PE-Cyanine5, clone: RA3-6B2, eBioscience™

Rat Monoclonal Antibody

Brand:  Affymetrix eBioscience 15-0452-83

Code : Z2

Additional Details : Weight : 0.25000kg

 View more versions of this product

Product Code. 15300490

  • £220.39 / 200µg


EU Stock 1
Add to basket

Description

Description

Description: The RA3-6B2 monoclonal antibody reacts with exon A-restricted isoform of mouse CD45, a 220kDa surface molecule. CD45R/B220 epitope is mainly expressed by the B cell lineage from early Pro-B to mature B cells. However, some activated T cells, lymphokine activated killer cells (LAK), NK cell progenitors in the bone marrow, and T cells of the \I{lpr/lpr\I} mutant mouse also express this antigen.Applications Reported: The RA3-6B2 antibody has been reported for use in flow cytometric analysis.Applications Tested: The RA3-6B2 antibody has been tested by flow cytometric analysis of mouse splenocytes. This can be used at less than or equal to 0.25 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Light sensitivity: This tandem dye is sensitive photo-induced oxidation. Please protect this vial and stained samples from light. Fixation: Samples can be stored in IC Fixation Buffer (cat. 00-8222) (100 μL cell sample + 100 μL IC Fixation Buffer) or 1-step Fix/Lyse Solution (cat. 00-5333) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically.Excitation: 488-561 nm; Emission: 667 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.Filtration: 0.2μm post-manufacturing filtered.
Specifications

Specifications

CD45R (B220)
Monoclonal
0.2mg/mL
PBS with 0.1% gelatin and 0.09% sodium azide; pH 7.2
P08575, P06800
B220, CD45R, Cd45, Ly-5, Lyt-4, T200, Cluster designation 45R
Rat
Affinity chromatography
RUO
Antibody
Monoclonal
Murine
Flow Cytometry
RA3-6B2
PE-Cyanine5
PTPRC
Conjugated
PTPRC
IgG2a, kappa
200μg
Store at 2-8°C. Do not freeze. Light-sensitive material.
Primary
5788
Videos

For Research Use Only.