CD45R (B220) Monoclonal Antibody (RA3-6B2), eFluor™ 615, eBioscience™, Invitrogen™

Rat Monoclonal Antibody

Product Code	Brand	Quantity
15321100	Affymetrix eBioscience 42-0452-82	100 μg
15311100	Affymetrix eBioscience 42-0452-80	25 μg

Description

Description: The RA3-6B2 monoclonal antibody reacts with exon A-restricted isoform of mouse CD45, a 220 kDa surface molecule. CD45R/B220 epitope is mainly expressed by the B cell lineage from early Pro-B to mature B cells. However, some activated T cells, lymphokine activated killer cells (LAK), NK cell progenitors in the bone marrow, and T cells of the lpr/lpr mutant mouse also express this antigen. Applications Reported: This RA3-6B2 antibody has been reported for use in immunohistochemical staining of frozen tissue sections (IHC-F), and immunocytochemistry. Applications Tested: This RA3-6B2 antibody has been tested by immunohistochemistry on frozen mouse spleen at less than or equal to 1 μg/mL. It is recommended that this antibody be carefully titrated for optimal performance in the assay of interest. This product has not been validated for flow cytometric analysis. Filter Recommendation: When using this eFluor™ 615 antibody conjugate, we recommend a filter that will capture the 615 emission wavelength (for example, Excitation 560/55, 585LP, Emission 645/75). A standard Alexa Fluor™ 594 filter is acceptable. Excitation: 595 nm; Emission: 615 nm. Filtration: 0.2 μm post-manufacturing filtered.

CD45R (B220) Monoclonal antibody specifically detects CD45R (B220) in Human, Mouse samples. It is validated for Immunocytochemistry, Immunohistochemistry (Frozen).

Specifications

• Antigen: CD45R (B220)
• Concentration: 0.2 mg/mL
• Conjugate: eFluor 615
• Host Species: Rat
• Target Species: Human, Mouse
• Gene Symbols: PTPRC
• Primary or Secondary: Primary
• Content And Storage: 4° C, store in dark, DO NOT FREEZE!
• Gene: PTPRC

• Clone: RA3-6B2
• Classification: Monoclonal
• Form: Liquid
• Regulatory Status: RUO
• Gene ID (Entrez): 19264, 5788
• Isotype: IgG2a κ
• Purification Method: Affinity chromatography
• Product Type: Antibody

For Research Use Only.