CD45R (B220), eFluor 615, clone: RA3-6B2, eBioscience™
Rat Monoclonal Antibody
Brand: Affymetrix eBioscience 42-0452-82
Code : Z2
Additional Details : Weight : 0.25000kg
DescriptionDescription: The RA3-6B2 monoclonal antibody reacts with exon A-restricted isoform of mouse CD45, a 220kDa surface molecule. CD45R/B220 epitope is mainly expressed by the B cell lineage from early Pro-B to mature B cells. However, some activated T cells, lymphokine activated killer cells (LAK), NK cell progenitors in the bone marrow, and T cells of the lpr/lpr mutant mouse also express this antigen.Applications Reported: This RA3-6B2 antibody has been reported for use in immunohistochemical staining of frozen tissue sections (IHC-F), and immunocytochemistry.Applications Tested: This RA3-6B2 antibody has been tested by immunohistochemistry on frozen mouse spleen at less than or equal to 1 μg/mL. It is recommended that this antibody be carefully titrated for optimal performance in the assay of interest. This product has not been validated for flow cytometric analysis.Filter Recommendation: When using this eFluor® 615 antibody conjugate, we recommend a filter that will capture the 615 emission wavelength (for example, Excitation 560/55, 585LP, Emission 645/75). A standard Alexa Fluor® 594 filter is acceptable.Excitation: 595 nm; Emission: 615 nm.Filtration: 0.2μm post-manufacturing filtered.
|PBS with 0.1% gelatin and 0.09% sodium azide; pH 7.2|
|B220, CD45R, Cd45, Ly-5, Lyt-4, T200, Cluster designation 45R|
|Store at 2-8°C. Do not freeze. Light-sensitive material.|
For Research Use Only.