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CD45R (B220), Functional Grade, clone: RA3-6B2, eBioscience™

Rat Monoclonal Antibody

Brand:  Affymetrix eBioscience 16-0452-85

Code : Z2

Additional Details : Weight : 0.01000kg

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Product Code. 15248457

  • £181.43 / 500µg


EU Stock 1
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Description

Description

Description: The RA3-6B2 monoclonal antibody reacts with exon A-restricted isoform of mouse CD45, a 220kDa surface molecule. CD45R/B220 epitope is mainly expressed by the B cell lineage from early Pro-B to mature B cells. However, some activated T cells, lymphokine activated killer cells (LAK), NK cell progenitors in the bone marrow, and T cells of the lpr/lpr mutant mouse also express this antigen.Applications Reported: The RA3-6B2 antibody has been reported for use in flow cytometric analysis. RA3-6B2 has also been reported in functional studies of B cells.Applications Tested: The RA3-6B2 antibody has been tested by flow cytometric analysis of mouse splenocytes and can be used at less than or equal to 0.5 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Endotoxin: Less than 0.001 ng/ug antibody as determined by the LAL assay.Storage and handling: Use in a sterile environment.Filtration: 0.2μm post-manufacturing filtered.Purity: Greater than 90%, as determined by SDS-PAGE.Endotoxin Level: Less than 0.001 ng/μg antibody, as determined by LAL assay.Aggregation: Less than 10%, as determined by HPLC.
Specifications

Specifications

CD45R (B220)
Monoclonal
1mg/mL
PBS with no preservative; pH 7.2
P06800, P08575
B220, CD45R, Cd45, Ly-5, Lyt-4, T200, Cluster designation 45R
Rat
Affinity chromatography
RUO
Antibody
Monoclonal
HumanþMurine
Flow CytometryþFunctional Assay
RA3-6B2
Functional Grade
PTPRC
Purified
PTPRC
IgG2a, kappa
500μg
Store at 2-8°C.
Primary
5788
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