CD45R (B220) Monoclonal Antibody (RA3-6B2), Alexa Fluor™ 700, eBioscience™, Invitrogen™

Rat Monoclonal Antibody

Brand:  Affymetrix eBioscience 56-0452-80

Product Code. 15302130

Description

Description: The RA3-6B2 monoclonal antibody reacts with exon A-restricted isoform of mouse CD45, a 220 kDa surface molecule. CD45R/B220 epitope is mainly expressed by the B cell lineage from early Pro-B to mature B cells. However, some activated T cells, lymphokine activated killer cells (LAK), NK cell progenitors in the bone marrow, and T cells of the lpr/lpr mutant mouse also express this antigen. Applications Reported: This RA3-6B2 antibody has been reported for use in flow cytometric analysis. Applications Tested: This RA3-6B2 antibody has been tested by flow cytometric analysis of mouse splenocytes. This can be used at less than or equal to 0.25 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. Alexa Fluor™ 700 emits at 723 nm and can be excited with the red laser (633 nm). Most instruments will require a 685 LP mirror and 710/20 filter. Please make sure that your instrument is capable of detecting this fluorochrome. Excitation: 633-647 nm; Emission: 723 nm; Laser: Red Laser. Filtration: 0.2 μm post-manufacturing filtered.

CD45R is a member of the protein tyrosine phosphatase (PTP) family. PTPs are known to be signaling molecules that regulate a variety of cellular processes including cell growth, differentiation, mitotic cycle, and oncogenic transformation. CD45R contains an extracellular domain, a single transmembrane segment and two tandem intracytoplasmic catalytic domains. It is specifically expressed in hematopoietic cells and has been shown to be an essential regulator of T- and B-cell antigen receptor signaling.

Specifications

• Antigen: CD45R
• Classification: Monoclonal
• Concentration: 0.2mg/mL
• Formulation: aqueous buffer, 0.09% sodium azide, may contain carrier protein/stabilizer
• Host Species: Rat
• Regulatory Status: RUO
• Target Species: Human, Mouse
• Isotype: IgG2a κ

• Applications: Flow Cytometry
• Clone: RA3-6B2
• Conjugate: Alexa Fluor 700
• Gene Alias: Ly-5, Lyt-4, T200
• Quantity: 25 μg
• Primary or Secondary: Primary
• Content And Storage: Store at 2-8°C. Do not freeze. Light-sensitive material.

For Research Use Only.