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CD45R (B220), Alexa Fluor 488, clone: RA3-6B2, eBioscience™

Rat Monoclonal Antibody

Brand:  Affymetrix eBioscience 53-0452-82

Code : Z2

Additional Details : Weight : 0.25000kg

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Product Code. 15302000

  • £133.91 / 100µg


EU Stock 2
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Description

Description

Description: The RA3-6B2 monoclonal antibody reacts with exon A-restricted isoform of mouse CD45, a 220kDa surface molecule. CD45R/B220 epitope is mainly expressed by the B cell lineage from early Pro-B to mature B cells. However, some activated T cells, lymphokine activated killer cells (LAK), NK cell progenitors in the bone marrow, and T cells of the lpr/lpr mutant mouse also express this antigen.Applications Reported: This RA3-6B2 antibody has been reported for use in immunohistochemical staining of frozen tissue sections, flow cytometric analysis, and immunohistochemical staining of formalin-fixed paraffin embedded tissue sections.Applications Tested: This RA3-6B2 antibody has been tested by flow cytometric analysis of mouse splenocytes. This can be used at less than or equal to 0.5 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. This RA3-6B2 antibody has also been tested by immunohistochemistry of frozen tissue sections and can be used at less than or equal to 20 μg/mL. The RA3-6B2 antibody has been tested by immunohistochemistry of formalin-fixed paraffin embedded tissue sections using low pH antigen retrieval and can be used at less than or equal to 20 μg/mL. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Excitation: 488 nm; Emission: 519 nm; Laser: Blue Laser.Filtration: 0.2μm post-manufacturing filtered.
Specifications

Specifications

CD45R
Monoclonal
0.5mg/mL
PBS with 0.1% gelatin and 0.09% sodium azide; pH 7.2
P06800, P08575
B220, CD45R, Cd45, Ly-5, Lyt-4, T200, Cluster designation 45R
Rat
Affinity chromatography
RUO
Antibody
Monoclonal
Murine
Immunohistochemistry (Frozen)
RA3-6B2
Alexa Fluor 488
PTPRC
Conjugated
Ptprc
IgG2a, kappa
100μg
Store at 2-8°C. Do not freeze. Light-sensitive material.
Primary
5788
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