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CD45, clone: 30-F11, eBioscience™

Rat Monoclonal Antibody

Brand:  Affymetrix eBioscience 14-0451-85

Code : Z2

Additional Details : Weight : 0.01000kg

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Product Code. 15226967

  • £115.56 / 500µg


Estimated Shipment
02-03-2018
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Disclaimers:

For Research Use Only.


Description

Description

Description: The 30-F11 monoclonal antibody reacts with all isoforms of mouse CD45, also known as Leukocyte Common Antigen (LCA). CD45 is expressed by all hematopoietic cells excluding mature erythrocytes and platelets. The cytoplasmic portion of CD45 has tyrosine phosphatase enzymatic activity and plays an important role in activation of lymphocytes.Applications Reported: The 30-F11 antibody has been reported for use in flow cytometric analysis, immunoprecipitation, and immunohistochemical staining. 30-F11 has also been reported in complement-dependent cytotoxicity. (Please use Functional Grade purified 30-F11 in functional assays.).Applications Tested: The 30-F11 antibody has been tested by flow cytometric analysis of mouse bone marrow cells and splenocytes. This can be used at less than or equal to 0.5 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Purity: Greater than 90%, as determined by SDS-PAGE.Aggregation: Less than 10%, as determined by HPLC.Filtration: 0.2μm post-manufacturing filtered.
Specifications

Specifications

CD45
30-F11
Unconjugated
Purified
Rat
500μg
Store at 2-8°C.
Monoclonal
Immunoprecipitation
0.5mg/mL
aqueous buffer, 0.09% sodium azide, may contain carrier protein/stabilizer
Leukocyte Common Antigen, LCA, Ly-5
IgG2b
RUO
Primary
Murine
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