missing translation for 'onlineSavingsMsg'
Learn More

CD45 Monoclonal Antibody (30-F11), Biotin, eBioscience™, Invitrogen™

Rat Monoclonal Antibody

£51.00 - £173.00

Specifications

Antigen CD45
Clone 30-F11
Concentration 0.5 mg/mL
Applications Flow Cytometry
Classification Monoclonal
View More Specs

Products 2
Product Code Brand Quantity Price Quantity & Availability  
Product Code Brand Quantity Price Quantity & Availability  
15279559
View Documents Promotion Details
Affymetrix eBioscience
13-0451-82
100 μg
£51.00
100µg
View alternative products
Save Up to 
»
In Stock
to see stock.
Please to purchase this item. Need a web account? Register with us today!
15289559
View Documents Promotion Details
Affymetrix eBioscience
13-0451-85
500 μg
£173.00
500µg
View alternative products
Save Up to 
»
In Stock
to see stock.
Please to purchase this item. Need a web account? Register with us today!
Description

Description

Description: The 30-F11 monoclonal antibody reacts with all isoforms of mouse CD45, also known as Leukocyte Common Antigen (LCA). CD45 is expressed by all hematopoietic cells excluding mature erythrocytes and platelets. The cytoplasmic portion of CD45 has tyrosine phosphatase enzymatic activity and plays an important role in activation of lymphocytes. Applications Reported: The 30-F11 antibody has been reported for use in flow cytometric analysis. Applications Tested: The 30-F11 antibody has been tested by flow cytometric analysis of mouse bone marrow cells and splenocytes. This can be used at less than or equal to 0.125 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. Filtration: 0.2 μm post-manufacturing filtered.

CD45 (LCA, leukocyte common antigen) is a receptor-type protein tyrosine phosphatase (PTP) ubiquitously expressed in all nucleated hematopoietic cells, comprising approximately 10% of all surface proteins in lymphocytes. CD45 is absent on non-hematopoietic cell lines, normal and malignant, non-hematopoietic tissues. CD45 glycoprotein is crucial in lymphocyte development and antigen signaling, serving as an important regulator of Src-family kinases. CD45 protein exists as multiple isoforms as a result of alternative splicing, differ in their extracellular domains but share identical transmembrane and cytoplasmic domains. CD45RA is an isoform of the CD45 complex and has restricted expression between different subtypes of lymphoid cells. CD45 isoforms differ in their ability to translocate into the glycosphingolipid-enriched membrane domains and their expression depends on cell type and physiological state of the cell. CD45 has been shown to be an essential regulator of T- and B-cell antigen receptor signaling and suppresses JAK kinases to regulate cytokine receptor signaling. CD45 is also important in promoting cell survival by modulating integrin-mediated signal transduction pathway, DNA fragmentation during apoptosis and inhibition or upregulation of various immunological functions.
Specifications

Specifications

CD45
0.5 mg/mL
Monoclonal
Liquid
RUO
P06800
IgG2b κ
Affinity chromatography
Antibody
30-F11
Flow Cytometry
Biotin
Rat
Mouse
19264
Primary
4° C, store in dark, DO NOT FREEZE!
Videos
SDS
Documents

Documents

Product Certifications

For Research Use Only.