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CD45, eFluor 450, clone: YKIX716.13, eBioscience™

Rat Monoclonal Antibody

Brand:  Affymetrix eBioscience 48-5450-41

Code : NEW

Additional Details : Weight : 0.23750kg

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Product Code. 15599756

  • £62.36 / 25 tests


EU Stock 2
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Description

Description

Description: This YKIX716.13 monoclonal antibody reacts with canine CD45, also known as the leukocyte common antigen. CD45 is expressed by all hematopoietic cells except red blood cells and platelets. CD45 exists as multiple isoforms due to alternative splicing. The C-terminal domain of CD45 exhibits tyrosine phosphatase activity that enables signaling by T cell and B cell receptors following activation.This monoclonal antibody recognizes all isoforms of CD45. In addition, when used in conjunction with YKIX753.22, this antibody has been reported to promote cell depletion via antibody-dependent cell-mediated cytotoxicity.Applications Reported: This YKIX716.13 antibody has been reported for use in flow cytometric analysis.Applications Tested: This YKIX716.13 antibody has been pre-titrated and tested by flow cytometric analysis of normal canine peripheral blood cells. This can be used at 5 μL (0.5 μg) per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. eFluor® 450 is an alternative to Pacific Blue®. eFluor® 450 emits at 445 nm and is excited with the Violet laser (405 nm). Please make sure that your instrument is capable of detecting this fluorochome.Excitation: 405 nm; Emission: 445 nm; Laser: Violet Laser.Filtration: 0.2μm post-manufacturing filtered.
Specifications

Specifications

CD45
Monoclonal
5μl/Test
PBS with 0.1% gelatin, 0.2% BSA and 0.09% sodium azide; pH 7.2
Conjugated
PTPRC
IgG2b, kappa
25 Tests
Store at 2-8°C. Do not freeze. Light-sensitive material.
Primary
Canine
Flow Cytometry
YKIX716.13
eFluor 450
PTPRC
Cluster designation 45, common leukocyte antigen, T220 and B220
Rat
Affinity chromatography
RUO
Antibody
Monoclonal
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