CD45 Monoclonal Antibody (YKIX716.13), eFluor™ 450, eBioscience™, Invitrogen™

Rat Monoclonal Antibody

Brand:  Affymetrix eBioscience 48-5450-42

Product Code. 15509766

Description

Description: This YKIX716.13 monoclonal antibody reacts with canine CD45, also known as the leukocyte common antigen. CD45 is expressed by all hematopoietic cells except red blood cells and platelets. CD45 exists as multiple isoforms due to alternative splicing. The C-terminal domain of CD45 exhibits tyrosine phosphatase activity that enables signaling by T cell and B cell receptors following activation. This monoclonal antibody recognizes all isoforms of CD45. In addition, when used in conjunction with YKIX753.22, this antibody has been reported to promote cell depletion via antibody-dependent cell-mediated cytotoxicity. Applications Reported: This YKIX716.13 antibody has been reported for use in flow cytometric analysis. Applications Tested: This YKIX716.13 antibody has been pre-titrated and tested by flow cytometric analysis of normal canine peripheral blood cells. This can be used at 5 μL (0.5 μg) per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. eFluor™ 450 is an alternative to Pacific Blue™. eFluor™ 450 emits at 445 nm and is excited with the Violet laser (405 nm). Please make sure that your instrument is capable of detecting this fluorochome. Excitation: 405 nm; Emission: 445 nm; Laser: Violet Laser. Filtration: 0.2 μm post-manufacturing filtered.

CD45 Monoclonal antibody specifically detects CD45 in Canine samples. It is validated for Flow Cytometry.

Specifications

• Antigen: CD45
• Classification: Monoclonal
• Concentration: 5 μL/Test
• Formulation: PBS with 0.2% BSA and 0.09% sodium azide; pH 7.2
• Gene Accession No.: 0
• Gene Symbols: PTPRC
• Purification Method: Affinity chromatography
• Regulatory Status: RUO
• Gene ID (Entrez): 490255
• Content And Storage: 4° C, store in dark, DO NOT FREEZE!
• Form: Liquid

• Applications: Flow Cytometry
• Clone: YKIX716.13
• Conjugate: eFluor 450
• Gene: PTPRC
• Gene Alias: B220; cd45; CD45 antigen; CD45 antigen isoform 1 precursor; CD45 antigen isoform 2 precursor; CD45 antigen isoform 3 precursor; CD45 antigen isoform 4 precursor; CD45 antigen isoform 5 precursor; CD45 antigen isoform 6 precursor; CD45R; GP180; Lca; L-CA; leucocyte common antigen; leukocyte common antigen; leukocyte common antigen A; leukocyte common antigen B; leukocyte common antigen, CD45; loc; LOW QUALITY PROTEIN: receptor-type tyrosine-protein phosphatase C; LY5; Ly-5; lymphocyte antigen 5; lymphocyte common antigen; Lyt-4; membrane tyrosine phosphatase; protein tyrosine phosphatase lambda; protein tyrosine phosphatase receptor type C; protein tyrosine phosphatase, receptor type C; protein tyrosine phosphatase, receptor type, C; protein tyrosine phosphatase, receptor type, c polypeptide; Protein tyrosine phosphatase, receptor-type, c polypeptide; protein tyrosine phosphatase; alternatively spliced; Ptprc; Receptor-type tyrosine-protein phosphatase C; RT7; T200; T200 glycoprotein; T
• Host Species: Rat
• Quantity: 100 Tests
• Primary or Secondary: Primary
• Target Species: Canine
• Product Type: Antibody
• Isotype: IgG2b κ

For Research Use Only.