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CD45 Mouse anti-Human, PerCP-Cyanine5.5, Clone: 2D1, eBioscience™

Mouse Monoclonal Antibody

Brand:  Affymetrix eBioscience 45-9459-41

Code : NEW

Additional Details : Weight : 0.23750kg

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Product Code. 15539046

  • £98.75 / 25 tests

Estimated Shipment: 28-09-2021

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Description

Description

Description: The 2D1 monoclonal antibody reacts with all isoforms of human CD45, also known as Leukocyte Common Antigen (LCA). CD45 is expressed by all hematopoietic cells excluding circulating erythrocytes and platelets. The cytoplasmic portion of CD45 has tyrosine phosphatase enzymatic activity and plays an important role in activation of lymphocytes. Applications Reported: This 2D1 antibody has been reported for use in flow cytometric analysis. Applications Tested: This 2D1 antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at 5 µL (0.125 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. Excitation: 488 nm; Emission: 695 nm; Laser: Blue Laser. Filtration: 0.2 μm post-manufacturing filtered.

CD45 (LCA, leukocyte common antigen) is a receptor-type protein tyrosine phosphatase (PTP) ubiquitously expressed in all nucleated hematopoietic cells, comprising approximately 10% of all surface proteins in lymphocytes. CD45 is absent on non-hematopoietic cell lines, normal and maligt, non-hematopoietic tissues. CD45 glycoprotein is crucial in lymphocyte development and antigen signaling, serving as an important regulator of Src-family kinases. CD45 protein exists as multiple isoforms as a result of alternative splicing, differ in their extracellular domains but share identical transmembrane and cytoplasmic domains. CD45RA is an isoform of the CD45 complex and has restricted expression between different subtypes of lymphoid cells. CD45 isoforms differ in their ability to translocate into the glycosphingolipid-enriched membrane domains and their expression depends on cell type and physiological state of the cell. CD45 has been shown to be an essential regulator of T- and B-cell antigen receptor signaling and suppresses JAK kinases to regulate cytokine receptor signaling. CD45 is also important in promoting cell survival by modulating integrin-mediated signal transduction pathway, DNA fragmentation during apoptosis and inhibition or upregulation of various immunological functions.
Specifications

Specifications

CD45
Monoclonal
5 μL/Test
PBS with 0.1% gelatin, 0.2% BSA and 0.09% sodium azide; pH 7.2
P08575
Cluster designation 45; common leukocyte antigen; T220 and B220; HCD45
Mouse
Affinity chromatography
RUO
Antibody
Monoclonal
Human
Flow Cytometry
2D1
PerCP-Cyanine5.5
PTPRC
Liquid
PTPRC
IgG1, kappa
25 Tests
4° C, store in dark, DO NOT FREEZE!
Primary
5788
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