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CD45, PE-Cyanine5.5, clone: HI30, eBioscience™

Mouse Monoclonal Antibody

Brand:  Affymetrix eBioscience 35-0459-42

Code : NEW

Additional Details : Weight : 0.23750kg

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Product Code. 15558856

  • £182.82 / 100 tests


EU Stock 3
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Disclaimers:

For Research Use Only.


Description

Description

Description: The HI30 monoclonal antibody reacts with all isoforms of human CD45, also known as Leukocyte Common Antigen (LCA). CD45 is expressed by all hematopoietic cells excluding circulating erythrocytes and platelets. The cytoplasmic portion of CD45 has tyrosine phosphatase enzymatic activity and plays an important role in activation of lymphocytes.Applications Reported: This HI30 antibody has been reported for use in flow cytometric analysis.Applications Tested: This HI30 antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at 5 μL (0.03 μg) per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. Light sensitivity: This tandem dye is sensitive photo-induced oxidation. Please protect this vial and stained samples from light. Fixation: Samples can be stored in IC Fixation Buffer (cat. 00-8222) (100 μL cell sample + 100 μL IC Fixation Buffer) or 1-step Fix/Lyse Solution (cat. 00-5333) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically.Excitation: 488-567 nm; Emission: 690 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser
Specifications

Specifications

CD45
HI30
PE-Cyanine5.5
Conjugated
Murine
100 Tests
Store at 2-8°C. Do not freeze. Light-sensitive material. This tandem dye is sensitive to photo-induced oxidation. Protect this vial from light during storage
Monoclonal
Flow Cytometry
5μL (0.03μg)/test
aqueous buffer, 0.09% sodium azide, may contain carrier protein/stabilizer
Leukocyte Common Antigen, LCA, Ly-5
IgG1
RUO
Primary
Human
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