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CD45, Alexa Fluor 532, clone: HI30, eBioscience™

Mouse Monoclonal Antibody

Brand:  Affymetrix eBioscience 58-0459-42

Code : NEW

Additional Details : Weight : 0.23750kg

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Product Code. 15570387

  • £195.81 / 100 tests


Estimated Shipment date
from Supplier 04-06-2018
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Description

Description

Description: The HI30 monoclonal antibody reacts with all isoforms of human CD45, also known as Leukocyte Common Antigen (LCA). CD45 is expressed by all hematopoietic cells excluding circulating erythrocytes and platelets. The cytoplasmic portion of CD45 has tyrosine phosphatase enzymatic activity and plays an important role in activation of lymphocytes.Applications Reported: This HI30 antibody has been reported for use in flow cytometric analysis.Applications Tested: This HI30 antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at 5 μL (0.06 μg) per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. Alexa Fluor® 532 is excited with the Green laser (532 nm) and emits at 561 nm. This cannot be used with the Yellow-Green laser (561 nm). We recommend using a 560/14 band pass filter. Please make sure that your instrument is capable of detecting this fluorochome.Excitation: 532 nm; Emission: 561 nm; Laser: Green Laser
Specifications

Specifications

CD45
Monoclonal
5μl/Test
PBS with 0.1% gelatin, 0.2% BSA and 0.09% sodium azide; pH 7.2
P08575
Cluster designation 45, common leukocyte antigen, T220 and B220
Murine
Affinity chromatography
RUO
Antibody
Monoclonal
Human
Flow Cytometry
HI30
Alexa Fluor 532
PTPRC
Conjugated
PTPRC
IgG1, kappa
100 Tests
Store at 2-8°C. Do not freeze. Light-sensitive material.
Primary
5788
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