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CD45.2, PE-Cyanine7, clone: 104, eBioscience™

Mouse Monoclonal Antibody

Brand:  Affymetrix eBioscience 25-0454-80

Code : NEW

Additional Details : Weight : 0.09500kg

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Product Code. 15528406

  • £55.00 / 25µg


EU Stock 2
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Description

Description

Description: The 104 monoclonal antibody reacts with the mouse CD45 molecule, the leukocyte common antigen (LCA) in CD45.2-expressing mouse strains. The strains that express CD45.2 include the most commonly used mouse strains C57BL/6, BALB/c, C58, DBA/1, DBA/2, C3H/He, CBA, 129, A and AKR. CD45.2 is expressed by all leukocytes in these strains.Applications Reported: This 104 antibody has been reported for use in flow cytometric analysis.Applications Tested: This 104 antibody has been tested by flow cytometric analysis of mouse splenocytes. This can be used at less than or equal to 0.5 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Light sensitivity: This tandem dye is sensitive to photo-induced oxidation. Please protect this vial and stained samples from light.Fixation: Samples can be stored in IC Fixation Buffer (cat. 00-8222) (100 μL of cell sample + 100 μL of IC Fixation Buffer) or 1-step Fix/Lyse Solution (cat. 00-5333) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically.Excitation: 488-561 nm; Emission: 775 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.Filtration: 0.2μm post-manufacturing filtered.
Specifications

Specifications

CD45.2
Monoclonal
0.2mg/mL
PBS with 0.1% gelatin and 0.09% sodium azide; pH 7.2
P06800
B220, CD45, CD45R, GP180, L-CA, LCA, LY5, T200, CD45 antigen, T200 glycoprotein, T200 leukocyte common antigen, protein tyrosine phosphatase, receptor type, c polypeptide, receptor-type tyrosine-protein phosphatase C, PTPRC
Murine
Affinity chromatography
RUO
Antibody
Monoclonal
Murine
Flow Cytometry
104
PE-Cyanine7
Ptprc
Conjugated
Ptprc
IgG2a, kappa
25μg
Store at 2-8°C. Do not freeze. Light-sensitive material. This tandem dye is sensitive to photo-induced oxidation. Protect this vial from light during storage, handling and experimental procedures.
Primary
19264
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