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CD45.1 Monoclonal Antibody (A20), PE-Cyanine7, eBioscience™, Invitrogen™

Mouse Monoclonal Antibody

£95.75 - £164.00

Specifications

Antigen CD45.1
Clone A20
Concentration 0.2 mg/mL
Applications Flow Cytometry
Classification Monoclonal
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Products 2
Product Code Brand Quantity Price Quantity & Availability  
Product Code Brand Quantity Price Quantity & Availability  
15320930
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Affymetrix eBioscience
25-0453-81
50 μg
£95.75
50µg
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Estimated Shipment: 16-04-2024
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15330930
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Affymetrix eBioscience
25-0453-82
100 μg
£164.00
100µg
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Description

Description

Description: The A20 monoclonal antibody reacts with the mouse CD45 molecule, the leukocyte common antigen (LCA) in CD45.1-expressing mouse strains. The strains that express CD45.1 include SJL/J, DA, STS/A and RIII. CD45.1 is expressed by all leukocytes in these strains. Applications Reported: The A20 antibody has been reported for use in flow cytometric analysis. Applications Tested: This A20 antibody has been tested by flow cytometric analysis of mouse splenocytes. This can be used at less than or equal to 0.5 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. Light sensitivity: This tandem dye is sensitive photo-induced oxidation. Please protect this vial and stained samples from light. Fixation: Samples can be stored in IC Fixation Buffer (cat. 00-8222) (100 μL cell sample + 100 μL IC Fixation Buffer) or 1-step Fix/Lyse Solution (cat. 00-5333) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically.

CD45.1 Monoclonal antibody specifically detects CD45.1 in Mouse samples. It is validated for Flow Cytometry.
Specifications

Specifications

CD45.1
0.2 mg/mL
Monoclonal
Liquid
RUO
PBS with 0.09% sodium azide; pH 7.2
B220; Cd45; CD45 antigen; CD45R; GP180; Lca; L-CA; Leukocyte common antigen; loc; LY5; Ly-5; Lymphocyte antigen 5; lymphocyte common antigen; Lyt-4; protein tyrosine phosphatase, receptor type C; protein tyrosine phosphatase, receptor type, C; protein tyrosine phosphatase, receptor type, c polypeptide; PTPRC; Receptor-type tyrosine-protein phosphatase C; RT7; T200; T200 glycoprotein; T200 leukocyte common antigen
Ptprc
Primary
4° C, store in dark, DO NOT FREEZE!
Ptprc
A20
Flow Cytometry
PE-Cyanine7
Mouse
Mouse
P06800
19264
IgG2a κ
Affinity chromatography
Antibody
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