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CD45.1, APC-eFluor 780, clone: A20, eBioscience™

Mouse Monoclonal Antibody

Brand:  Affymetrix eBioscience 47-0453-80

Code : Z2

Additional Details : Weight : 0.25000kg

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Product Code. 15331630

  • £68.80 / 25µg


EU Stock 3
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Description

Description

Description: The A20 monoclonal antibody reacts with the mouse CD45 molecule, the leukocyte common antigen (LCA) in CD45.1-expressing mouse strains. The strains that express CD45.1 include SJL/J, DA, STS/A and RIII. CD45.1 is expressed by all leukocytes in these strains.Applications Reported: This A20 antibody has been reported for use in flow cytometric analysis.Applications Tested: This A20 antibody has been tested by flow cytometric analysis of mouse splenocytes. This can be used at less than or equal to 1 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.APC-eFluor® emits at 780 nm and is excited with the Red laser (633 nm). Please make sure that your instrument is capable of detecting this fluorochome.Light sensitivity: This tandem is sensitive to photo-induced oxidation. Please protect this vial and stained samples from light. Fixation: Samples can be stored in IC Fixation Buffer (cat. 00-8222) (100 μL cell sample + 100 μL IC Fixation Buffer) or 1-step Fix/Lyse Solution (cat. 00-5333) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically.Excitation: 633-647 nm; Emission: 780 nm; Laser: Red Laser.Filtration: 0.2μm post-manufacturing filtered.
Specifications

Specifications

CD45.1
Monoclonal
0.2mg/mL
PBS with 0.09% sodium azide; pH 7.2
P06800
CD45, L-CA, Lca, RT7, T200
Murine
Affinity chromatography
RUO
Antibody
Monoclonal
Murine
Flow Cytometry
A20
APC-eFluor 780
Ptprc
Conjugated
Ptprc
IgG2a, kappa
25μg
Store at 2-8°C. Do not freeze. Light-sensitive material. This tandem dye is sensitive to photo-induced oxidation. Protect this vial from light during storage, handling and experimental procedures.
Primary
19264
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