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CD44, eVolve 605, clone: IM7, eBioscience™

Rat Monoclonal Antibody

Brand:  Affymetrix eBioscience 83-0441-42

Code : NEW

Additional Details : Weight : 0.23750kg

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Product Code. 15510757

This item has been discontinued and is no longer available. View the product for possible alternatives or contact our Product Support team on 01509 555888 for assistance


Description

Description

Description: The IM7 monoclonal antibody reacts with all isoforms of mouse CD44 (Pgp-1). CD44 is expressed by hematopoietic and non-hematopoietic cells. Bone marrow myeloid cells and memory T cells highly express this antigen and peripheral B and T cells can upregulate the expression of CD44. CD44 functions as an adhesion molecule through its binding to hyaluronate, an extracellular matrix component.Applications Reported: This IM7 antibody has been reported for use in flow cytometric analysis.Applications Tested: This IM7 antibody has been pre-titrated and tested by flow cytometric analysis of mouse splenocytes. This can be used at 5 μL per test. A test is defined as the amount of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. For best results when using antibodies conjugated to eVolve™, we recommend a quick spin of the vial before use and avoid touching the bottom of the vial with the pipet tip.Laser/Filter Recommendation: eVolve™ 605 can be optimally excited with laser lines from 325-405 nm, and emits near 610 nm. We recommend using the violet (405 nm) laser line for excitation and a 595LP with a 605/40 or 610/20 bandpass filter, or equivalent, for detection.Fixation Recommendation: Antibodies conjugated to eVolve™ are compatible with eBioscience's fixation and permeabilization buffers.Excitation: 350-590 nm; Emission: 605 nm; Laser: Ultraviolet Laser, Violet LaserStorage and handling: For best results when using antibodies conjugated to eVolve™, we recommend a quick spin of the vial before use and avoid touching the bottom of the vial with the pipet tip.
Specifications

Specifications

CD44
Monoclonal
5μl/Test
PBS with 0.2% BSA and 0.09% sodium azide; pH 7.2
P15379, P16070
CD44 antigen, Cell surface glycoprotein CD44, hyaluronate binding protein, ECMR-III, GP90 lymphocyte homing adhesion receptor, HUTCH-I, PGP-1, extracellular matrix receptor III, hermes antigen, hyaluronate receptor, phagocytic glycoprotein 1, phagocytic glycoprotein I
Rat
Affinity chromatography
RUO
Antibody
Monoclonal
HumanĂ¾Murine
Flow Cytometry
IM7
eVolve 605
CD44
Conjugated
Cd44
IgG2b, kappa
100 Tests
Store at 2-8°C. Do not freeze. Light-sensitive material. This product should be used within 6 months from the date of receipt.
Primary
960
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