CD44, clone: IM7, eBioscience™
Rat Monoclonal Antibody
Brand: Affymetrix eBioscience 14-0441-85
Code : Z2
Additional Details : Weight : 0.01000kg
DescriptionDescription: The IM7 monoclonal antibody reacts with all isoforms of mouse CD44 (Pgp-1). CD44 is expressed by hematopoietic and non-hematopoietic cells. Bone marrow myeloid cells and memory T cells highly express this antigen and peripheral B and T cells can upregulate the expression of CD44. CD44 functions as an adhesion molecule through its binding to hyaluronate, an extracellular matrix component.Applications Reported: The IM7 antibody has been reported for use in flow cytometric analysis, immunoprecipitation, immunohistochemical staining and immunoblotting (non-reducing conditions). It has also been reported in complement-dependent cytotoxicity. (Please use Functional Grade purified IM7, cat. 16-0441, in functional assays.).Applications Tested: The IM7 antibody has been tested by flow cytometric analysis of mouse bone marrow cells and splenocytes. This can be used at less than or equal to 0.125 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Purity: Greater than 90%, as determined by SDS-PAGE.Aggregation: Less than 10%, as determined by HPLC.Filtration: 0.2μm post-manufacturing filtered.
|PBS with 0.09% sodium azide; pH 7.2|
|CD44 antigen, Cell surface glycoprotein CD44, hyaluronate binding protein, ECMR-III, GP90 lymphocyte homing adhesion receptor, HUTCH-I, PGP-1, extracellular matrix receptor III, hermes antigen, hyaluronate receptor, phagocytic glycoprotein 1, phagocytic glycoprotein I|
|Flow CytometryþFunctional AssayþImmunohistochemistryþImmunoprecipitationþWestern Blot|
|Store at 2-8°C.|
For Research Use Only.