CD43 Rat anti-Mouse, PE, Clone: eBioR2/60, eBioscience™

Rat Monoclonal Antibody

Overview
Brand: Affymetrix eBioscience

Manufacturer Part Number: 12-0431-83

UNSPSC: 12352200

Code: Z2

Additional Details:
Additional Details: Weight: 0.25000kg



Disclaimers: For Research Use Only.

Product Code. 15298529

Quantity Price
1 £ 176.7 / 200µg
Estimated Shipment date
from Supplier 04-05-2017
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Description and Specification

Specification

Antigen CD43
Formulation aqueous buffer, 0.09% sodium azide, may contain carrier protein/stabilizer
Isotype IgM
Storage Requirements Store at 2-8°C. Do not freeze. Light-sensitive material.
Applications Flow Cytometry
Quantity 200μg
Species Reactivity Mouse
Host Species Rat
Format Conjugated
Clone eBioR2/60
Gene Alias Leukosialin, LSN, Sialophorin, SPN
Regulatory Status RUO
Monoclonal or Polyclonal Monoclonal
Primary or Secondary Primary
Concentration 0.2mg/mL
Conjugate PE

The eBioR2/60 monoclonal antibody reacts with mouse CD43, also known as leukosialin and sialophorin. CD43 has glycoforms of approximately 115 and 130 kDa, and is expressed by an early B cell subset in BM, thymocytes and mature T cells, NK cells, myeloid lineage cells and platelets. CD43 is reported to bind to CD54 and play a role in adhesion and B cell survival.

While the S7 and 1B11 monoclonal antibody clones detect only certain isoforms of CD43, data suggests that R2/60 detects the expression of both isoforms. It has been demonstrated that R2/60 stains the majority of CD4+ and CD8+ splenocytes, as well as a significant population of CD19+ splenocytes. However, the clones S7 and 1B11 stain only CD4+ and CD8+, or CD19+ splenocytes, respectively. This suggests that S7 and 1B11 recognize distinct epitopes which are differentially expressed by splenic B and T cells, whereas R2/60 recognizes an epitope shared by both isoforms of CD43.