CD43, clone: eBioR2/60, eBioscience™
Rat Monoclonal Antibody
Brand: Affymetrix eBioscience 14-0431-82
Code : Z2
Additional Details : Weight : 0.01000kg
DescriptionDescription: The eBioR2/60 monoclonal antibody reacts with mouse CD43, also known as leukosialin and sialophorin. CD43 has glycoforms of approximately 115 and 130 kDa, and is expressed by an early B cell subset in BM, thymocytes and mature T cells, NK cells, myeloid lineage cells and platelets. CD43 is reported to bind to CD54 and play a role in adhesion and B cell survival. While the S7 and 1B11 monoclonal antibody clones detect only certain isoforms of CD43, data suggests that R2/60 detects the expression of both isoforms. It has been demonstrated that R2/60 stains the majority of CD4+ and CD8+ splenocytes, as well as a significant population of CD19+ splenocytes. However, the clones S7 and 1B11 stain only CD4+ and CD8+, or CD19+ splenocytes, respectively. This suggests that S7 and 1B11 recognize distinct epitopes which are differentially expressed by splenic B and T cells, whereas R2/60 recognizes an epitope shared by both isoforms of CD43.Applications Reported: This eBioR2/60 antibody has been reported for use in flow cytometric analysis, immunoprecipitation, and immunoblotting (WB). (Please use Functional Grade eBioR2/60 (cat. 16-0431) in functional assays.).Applications Tested: This eBioR2/60 antibody has been tested by flow cytometric analysis of mouse splenocytes. This can be used at less than or equal to 0.5 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Purity: Greater than 90%, as determined by SDS-PAGE.Aggregation: Less than 10%, as determined by HPLC.Filtration: 0.2μm post-manufacturing filtered.
|PBS with 0.09% sodium azide; pH 7.2|
|CD43, GALGP, GPL115, LSN, galactoglycoprotein, leukocyte sialoglycoprotein, leukosialin, sialophorin gpL115, leukosialin, CD43|
|Flow CytometryþImmunoprecipitationþWestern Blot|
|Store at 2-8°C.|
For Research Use Only.