CD43 Rat anti-Mouse, Clone: eBioR2/60, eBioscience™

Rat Monoclonal Antibody

Overview
Brand: Affymetrix eBioscience

Manufacturer Part Number: 14-0431-82

100UG:ANTI-MOUSE CD43 PURIFIED 100 UG

UNSPSC: 12352200

Code: Z2

Additional Details:
Additional Details: Weight: 0.01000kg



Disclaimers: For Research Use Only.

Product Code. 15216957

Quantity Price
1 £ 66.65 / 100µg
Estimated Shipment date
from Supplier 31-03-2017
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Description and Specification

Specification

Antigen CD43
Format Purified
Formulation aqueous buffer, 0.09% sodium azide, may contain carrier protein/stabilizer
Gene Alias Leukosialin, LSN, Sialophorin, SPN
Host Species Rat
Applications Flow Cytometry
Applications Immunoprecipitation
Applications Western Blotting
Isotype IgM
Quantity 100μg
Regulatory Status RUO
Species Reactivity Mouse
Storage Requirements Store at 2-8°C.
Primary or Secondary Primary
Monoclonal or Polyclonal Monoclonal
Clone eBioR2/60
Concentration 0.5mg/mL
Conjugate Unlabeled

The eBioR2/60 monoclonal antibody reacts with mouse CD43, also known as leukosialin and sialophorin. CD43 has glycoforms of approximately 115 and 130 kDa, and is expressed by an early B cell subset in BM, thymocytes and mature T cells, NK cells, myeloid lineage cells and platelets. CD43 is reported to bind to CD54 and play a role in adhesion and B cell survival.

While the S7 and 1B11 monoclonal antibody clones detect only certain isoforms of CD43, data suggests that R2/60 detects the expression of both isoforms. It has been demonstrated that R2/60 stains the majority of CD4+ and CD8+ splenocytes, as well as a significant population of CD19+ splenocytes. However, the clones S7 and 1B11 stain only CD4+ and CD8+, or CD19+ splenocytes, respectively. This suggests that S7 and 1B11 recognize distinct epitopes which are differentially expressed by splenic B and T cells, whereas R2/60 recognizes an epitope shared by both isoforms of CD43.