CD43 Rat anti-Mouse, Biotin, Clone: eBioR2/60, eBioscience™

Rat Monoclonal Antibody

Overview
Brand: Affymetrix eBioscience

Manufacturer Part Number: 13-0431-85

UNSPSC: 12352200

Code: Z2

Additional Details:
Additional Details: Weight: 0.25000kg



Disclaimers: For Research Use Only.

Product Code. 15299549

Quantity Price
1 £ 146.13 / 500µg
Estimated Shipment date
from Supplier 02-03-2017
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Description and Specification

Specification

Storage Requirements Store at 2-8°C. Do not freeze.
Clone eBioR2/60
Conjugate Biotin
Monoclonal or Polyclonal Monoclonal
Quantity 500μg
Format Conjugated
Gene Alias Leukosialin, LSN, Sialophorin, SPN
Regulatory Status RUO
Species Reactivity Mouse
Host Species Rat
Isotype IgM
Primary or Secondary Primary
Concentration 0.5mg/mL
Formulation aqueous buffer, 0.09% sodium azide, may contain carrier protein/stabilizer
Antigen CD43
Applications Flow Cytometry

The eBioR2/60 monoclonal antibody reacts with mouse CD43, also known as leukosialin and sialophorin. CD43 has glycoforms of approximately 115 and 130 kDa, and is expressed by an early B cell subset in BM, thymocytes and mature T cells, NK cells, myeloid lineage cells and platelets. CD43 is reported to bind to CD54 and play a role in adhesion and B cell survival.

While the S7 and 1B11 monoclonal antibody clones detect only certain isoforms of CD43, data suggests that R2/60 detects the expression of both isoforms. It has been demonstrated that R2/60 stains the majority of CD4+ and CD8+ splenocytes, as well as a significant population of CD19+ splenocytes. However, the clones S7 and 1B11 stain only CD4+ and CD8+, or CD19+ splenocytes, respectively. This suggests that S7 and 1B11 recognize distinct epitopes which are differentially expressed by splenic B and T cells, whereas R2/60 recognizes an epitope shared by both isoforms of CD43.