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CD42d, PerCP-eFluor™ 710, clone: 1C2, eBioscience™

Armenian Hamster Monoclonal Antibody

Brand:  Affymetrix eBioscience 46-0421-80

Code : Z2

Additional Details : Weight : 0.25000kg

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Product Code. 15301260

  • £77.91 / 25µg


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Description

Description

Description: The monoclonal antibody 1C2 recognizes mouse and rat CD42d, a single transmembrane protein also known as platelet glycoprotein V (GPV). CD42d interacts with CD42a, CD42b and CD42c to form the receptor for von willebrand factor and thrombin. This functional complex plays a role in platelet adhesion. Expression of CD42d (unlike other markers such as CD41) is restricted to resting platelets and megakaryocytes and therefore is a good marker for development of megakaryocytes.Applications Reported: This 1C2 antibody has been reported for use in flow cytometric analysis.Applications Tested: This 1C2 antibody has been tested by flow cytometric analysis of mouse platelets. This can be used at less than or equal to 0.25 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.PerCP-eFluor® 710 emits at 710 nm and is excited with the blue laser (488 nm); it can be used in place of PerCP-Cyanine5.5. We recommend using a 710/50 bandpass filter, however, the 695/40 bandpass filter is an acceptable alternative. Please make sure that your instrument is capable of detecting this fluorochrome.Fixation: Samples can be stored in IC Fixation Buffer (cat. 00-8222) (100 μL cell sample + 100 μL IC Fixation Buffer) or 1-step Fix/Lyse Solution (cat. 00-5333) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically.Excitation: 488 nm; Emission: 710 nm; Laser: Blue Laser.Filtration: 0.2μm post-manufacturing filtered.
Specifications

Specifications

CD42d
Monoclonal
0.2mg/mL
PBS with 0.1% gelatin and 0.09% sodium azide; pH 7.2
O08742, O08770
Platelets GP V, CD42d, Glycoprotein 5, GPV
Armenian Hamster
Affinity chromatography
RUO
Antibody
Monoclonal
Rat
Flow Cytometry
1C2
PerCP-eFluor 710
Gp5
Conjugated
Gp5
IgG
25μg
Store at 2-8°C. Do not freeze. Light-sensitive material.
Primary
14729
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