CD41a Mouse anti-Human, eFluor™ 450, Clone: HIP8, eBioscience™
Mouse Monoclonal Antibody
Brand: Affymetrix eBioscience 48-0419-41
Code : NEW
Additional Details : Weight : 0.23750kg
Description: The HIP8 monoclonal antibody reacts with the human CD41 molecule, the integrin alpha IIb also known as platelet GPIIb. CD41 non-covalently associates with integrin beta3 (GPIIIa, CD61) and is expressed by megakaryocytes and platelets. The CD41/CD61 complex is a receptor for fibronectin, fibrinogen, von Willebrand factor, vitronectin and thrombospondin and mediates platelets aggregation. HIP8 blocks platelet aggregation. Applications Reported: This HIP8 antibody has been reported for use in flow cytometric analysis. Applications Tested: This HIP8 antibody has been pre-titrated and tested by flow cytometric analysis of human platelets. This can be used at 5 µL (0.5 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. eFluor™ 450 is an alternative to Pacific Blue™. eFluor™ 450 emits at 445 nm and is excited with the Violet laser (405 nm). Please make sure that your instrument is capable of detecting this fluorochome. Excitation: 405 nm; Emission: 445 nm; Laser: Violet Laser. Filtration: 0.2 μm post-manufacturing filtered.CD41 (platelet glycoprotein IIb, ITGA2B) is composed of two subunits -120 kDa a, alpha and 23 kDa b, beta- that interact with CD61 in the presence of calcium to form a functional adhesive protein receptor. CD41 is also involved in blood coagulation by mediating platelet aggregation. Upon blood vessel damage, CD41 binds to a variety of proteins including von Willebrand factor, fibrinogen, fibronectin and vitronectin. CD41 is mainly expressed on megakaryocyte-platelet lineage, but generally belongs to the antigens that are expressed during the early stages of hematopoietic differentiation. Diseases associated with CD41 dysfunction include Glansmann Thrombasthenia, and Platelet type-16 bleeding disorder.
|PBS with 0.1% gelatin, 0.2% BSA and 0.09% sodium azide; pH 7.2|
|BDPLT2, CD41, CD41B, GP2B, GPIIb, GT, GTA, HPA3, GPalpha IIb, integrin alpha-IIb, platelet fibrinogen receptor, alpha subunit, platelet membrane glycoprotein IIb, platelet-specific antigen BAK, ITGA2B|
|4° C, store in dark, DO NOT FREEZE!|
For Research Use Only.