CD40 Monoclonal Antibody (1C10), Super Bright™ 600, eBioscience™, Invitrogen™

Rat Monoclonal Antibody

Product Code	Brand	Quantity
15953504	Affymetrix eBioscience 63-0401-82	100 μg

Description

Description: The 1C10 monoclonal antibody reacts with mouse CD40, a 45-50 kDa type I transmembrane glycoprotein. CD40 is a member of the TNFR family and is expressed by mouse B lymphocytes, follicular dendritic cells, thymic epithelium, and a subset of peripheral T cells. CD40 regulates B cell development/maturation by inducing Ig isotype switching and in combination with other signals such as IL-4, protects B cells from surface Ig-induced apoptosis and promotes proliferation. Interaction of CD40 with CD154 (gp39), its ligand on T cells, is important in T-B cell crosstalk and plays a role in costimulation and immune regulation. The monoclonal antibody 1C10 is reported to have agonistic activity in vitro and in vivo. Applications Reported: The 1C10 antibody has been reported for use in flow cytometric analysis. Applications Tested: The 1C10 antibody has been tested by flow cytometric analysis of mouse splenocytes. This can be used at less than or equal to 0.5 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. Super Bright 600 is a tandem dye that can be excited with the violet laser line (405 nm) and emits at 600 nm. We recommend using a 610/20 bandpass filter.

CD40 Monoclonal antibody specifically detects CD40 in Mouse samples. It is validated for Flow Cytometry.

Specifications

• Antigen: CD40
• Concentration: 0.2 mg/mL
• Classification: Monoclonal
• Form: Liquid
• Regulatory Status: RUO
• Gene Accession No.: P27512
• Isotype: IgG2a κ
• Purification Method: Affinity chromatography
• Product Type: Antibody

• Clone: 1C10
• Applications: Flow Cytometry
• Conjugate: Super Bright 600
• Host Species: Rat
• Target Species: Mouse
• Gene ID (Entrez): 21939
• Primary or Secondary: Primary
• Content And Storage: 4° C, store in dark, DO NOT FREEZE!