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CD40 Monoclonal Antibody (1C10), Super Bright™ 436, eBioscience™, Invitrogen™

Rat Monoclonal Antibody

£129.00 - £305.00

Specifications

Antigen CD40
Clone 1C10
Concentration 0.2 mg/mL
Applications Flow Cytometry
Classification Monoclonal
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Products 2
Product Code Brand Quantity Price Quantity & Availability  
Product Code Brand Quantity Price Quantity & Availability  
15963374
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Affymetrix eBioscience
62-0401-82
100 μg
£305.00
100µg
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15953374
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Affymetrix eBioscience
62-0401-80
25 μg This item is currently unavailable or has been discontinued.
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Description

Description

Description: The 1C10 monoclonal antibody reacts with mouse CD40, a 45-50 kDa type I transmembrane glycoprotein. CD40 is a member of the TNFR family and is expressed by mouse B lymphocytes, follicular dendritic cells, thymic epithelium, and a subset of peripheral T cells. CD40 regulates B cell development/maturation by inducing Ig isotype switching and in combination with other signals such as IL-4, protects B cells from surface Ig-induced apoptosis and promotes proliferation. Interaction of CD40 with CD154 (gp39), its ligand on T cells, is important in T-B cell crosstalk and plays a role in costimulation and immune regulation. The monoclonal antibody 1C10 is reported to have agonistic activity in vitro and in vivo. Applications Reported: The 1C10 antibody has been reported for use in flow cytometric analysis. Applications Tested: The 1C10 antibody has been tested by flow cytometric analysis of mouse splenocytes. This can be used at less than or equal to 0.5 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. Super Bright 436 can be excited with the violet laser line (405 nm) and emits at 436 nm. We recommend using a 450/50 bandpass filter, or equivalent.

CD40 Monoclonal antibody specifically detects CD40 in Mouse samples. It is validated for Flow Cytometry.
Specifications

Specifications

CD40
0.2 mg/mL
Monoclonal
Liquid
RUO
P27512
IgG2a κ
Affinity chromatography
Antibody
1C10
Flow Cytometry
Super Bright 436
Rat
Mouse
21939
Primary
4° C, store in dark, DO NOT FREEZE!
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Documents

Product Certifications