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CD40, PerCP-eFluor™ 710, clone: 1C10, eBioscience™

Rat Monoclonal Antibody

Brand:  Affymetrix eBioscience 46-0401-82

Code : NEW

Additional Details : Weight : 0.09500kg

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Product Code. 15569116

  • £209.17 / 100µg


EU Stock 3
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Description

Description

Description: The 1C10 monoclonal antibody reacts with mouse CD40, a 45-50kDa type I transmembrane glycoprotein. CD40 is a member of the TNFR family and is expressed by mouse B lymphocytes, follicular dendritic cells, thymic epithelium, and a subset of peripheral T cells. CD40 regulates B cell development/maturation by inducing Ig isotype switching and in combination with other signals such as IL-4, protects B cells from surface Ig-induced apoptosis and promotes proliferation. Interaction of CD40 with CD154 (gp39), its ligand on T cells, is important in T-B cell crosstalk and plays a role in costimulation and immune regulation.The monoclonal antibody 1C10 is reported to have agonistic activity in vitro and in vivo.Applications Reported: The 1C10 antibody has been reported for use in flow cytometric analysis.Applications Tested: This 1C10 antibody has been tested by flow cytometric analysis of mouse splenocytes. This can be used at less than or equal to 0.5 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.PerCP-eFluor® 710 emits at 710 nm and is excited with the blue laser (488 nm); it can be used in place of PerCP-Cyanine5.5. We recommend using a 710/50 bandpass filter, however, the 695/40 bandpass filter is an acceptable alternative. Please make sure that your instrument is capable of detecting this fluorochrome.Fixation: Samples can be stored in IC Fixation Buffer (cat. 00-8222) (100 μL cell sample + 100 μL IC Fixation Buffer) or 1-step Fix/Lyse Solution (cat. 00-5333) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically.Excitation: 488 nm; Emission: 710 nm; Laser: Blue Laser.Filtration: 0.2μm post-manufacturing filtered.
Specifications

Specifications

CD40
Monoclonal
0.2mg/mL
PBS with 0.1% gelatin and 0.09% sodium azide; pH 7.2
P27512
Bp50, CDW40, MGC9013, TNFRSF5, p50
Rat
Affinity chromatography
RUO
Antibody
Monoclonal
Murine
Flow Cytometry
1C10
PerCP-eFluor 710
Cd40
Conjugated
Cd40
IgG2a, kappa
100μg
Store at 2-8°C. Do not freeze. Light-sensitive material.
Primary
21939
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