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CD40, Functional Grade, clone: 1C10, eBioscience™

Rat Monoclonal Antibody

Brand:  Affymetrix eBioscience 16-0401-86

Code : Z2

Additional Details : Weight : 0.01000kg

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Product Code. 15288437

  • £346.94 / 1mg


EU Stock 4
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Description

Description

Description: The 1C10 monoclonal antibody reacts with mouse CD40, a 45-50kDa type I transmembrane glycoprotein. CD40 is a member of the TNFR family and is expressed by mouse B lymphocytes, follicular dendritic cells, thymic epithelium, and a subset of peripheral T cells. CD40 regulates B cell development/maturation by inducing Ig isotype switching and in combination with other signals such as IL-4, protects B cells from surface Ig-induced apoptosis and promotes proliferation. Interaction of CD40 with CD154 (gp39), its ligand on T cells, is important in T-B cell crosstalk and plays a role in costimulation and immune regulation.The monoclonal antibody 1C10 is reported to have agonistic activity in vitro and in vivo.Applications Reported: The 1C10 antibody has been reported for use in flow cytometric analysis. It has also been reported in in vitro activation of B cells.Applications Tested: The 1C10 antibody has been tested by flow cytometric analysis of mouse splenocytes. This can be used at less than or equal to 1 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Endotoxin: Less than 0.001 ng/ug antibody as determined by the LAL assay.Storage and handling: Use in a sterile environment.Filtration: 0.2μm post-manufacturing filtered.Purity: Greater than 90%, as determined by SDS-PAGE.Endotoxin Level: Less than 0.001 ng/μg antibody, as determined by LAL assay.Aggregation: Less than 10%, as determined by HPLC.
Specifications

Specifications

CD40
Monoclonal
1mg/mL
PBS with no preservative; pH 7.2
P27512
Bp50, CDW40, MGC9013, TNFRSF5, p50
Rat
Affinity chromatography
RUO
Antibody
Monoclonal
Murine
Functional Assay
1C10
Functional Grade
Cd40
Purified
Cd40
IgG2a, kappa
1mg
Store at 2-8°C.
Primary
21939
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