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CD40 Monoclonal Antibody (5C3), PerCP-eFluor™ 710, eBioscience™, Invitrogen™
Mouse Monoclonal Antibody
£274.00
Specifications
Antigen | CD40 |
---|---|
Clone | 5C3 |
Concentration | 5 μL/Test |
Applications | Flow Cytometry |
Classification | Monoclonal |
Description
Description: The 5C3 monoclonal antibody reacts with human CD40, a 45-50 kDa type I transmembrane glycoprotein. CD40 is a member of the TNFR family and is expressed by B lymphocytes, follicular dendritic cells, thymic epithelium, and a subset of peripheral T cells. CD40 regulates B cell development and maturation by inducing Ig isotype-switching and in combination with other signals such as IL-4, protects B cells from surface Ig-induced apoptosis and promotes proliferation. Interaction of CD40 with CD154 (gp39), its ligand on T cells, is important in T-B cell crosstalk and plays a role in costimulation and immune regulation. 5C3 is reported to be used for activation of B cells in in vitro functional assays. Applications Reported: This 5C3 antibody has been reported for use in flow cytometric analysis. Applications Tested: This 5C3 antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at 5 μL (0.03 μg) per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. PerCP-eFluor™ 710 emits at 710 nm and is excited with the blue laser (488 nm); it can be used in place of PerCP-Cyanine5.5. We recommend using a 710/50 bandpass filter, however, the 695/40 bandpass filter is an acceptable alternative.
CD40 Monoclonal antibody specifically detects CD40 in Human samples. It is validated for Flow Cytometry.Specifications
CD40 | |
5 μL/Test | |
Monoclonal | |
Liquid | |
RUO | |
P25942 | |
CD40 | |
Primary | |
4° C, store in dark, DO NOT FREEZE! | |
CD40 |
5C3 | |
Flow Cytometry | |
PerCP-eFluor 710 | |
Mouse | |
Human | |
958 | |
IgG1 κ | |
Affinity chromatography | |
Antibody |
For Research Use Only.