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CD40, eFluor 450, clone: 5C3, eBioscience™

Mouse Monoclonal Antibody

Brand:  Affymetrix eBioscience 48-0409-42

Code : NEW

Additional Details : Weight : 0.23750kg

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Product Code. 15529676

  • £174.66 / 100 tests

EU Stock 2
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Description: The 5C3 monoclonal antibody reacts with human CD40, a 45-50kDa type I transmembrane glycoprotein. CD40 is a member of the TNFR family and is expressed by B lymphocytes, follicular dendritic cells, thymic epithelium, and a subset of peripheral T cells. CD40 regulates B cell development and maturation by inducing Ig isotype-switching and in combination with other signals such as IL-4, protects B cells from surface Ig-induced apoptosis and promotes proliferation. Interaction of CD40 with CD154 (gp39), its ligand on T cells, is important in T-B cell crosstalk and plays a role in costimulation and immune regulation. 5C3 is reported to be used for activation of B cells in in vitro functional assays.Applications Reported: This 5C3 antibody has been reported for use in flow cytometric analysis.Applications Tested: This 5C3 antibody has been pre-titrated and tested by flow cytometric analyis of normal human peripheral blood cells. This can be used at 5 μL (0.125 μg) per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. eFluor® 450 is an alternative to Pacific Blue®. eFluor® 450 emits at 445 nm and is excited with the Violet laser (405 nm). Please make sure that your instrument is capable of detecting this fluorochome.Excitation: 405 nm; Emission: 445 nm; Laser: Violet Laser.Filtration: 0.2μm post-manufacturing filtered.


eFluor 450
100 Tests
Store at 2-8°C. Do not freeze. Light-sensitive material.
Flow Cytometry
5μL (0.125μg)/test
aqueous buffer, 0.09% sodium azide, may contain carrier protein/stabilizer
IgG1, kappa

For Research Use Only.