CD40, FITC, clone: HM40-3, eBioscience™

Armenian Hamster Monoclonal Antibody

Brand:  Affymetrix eBioscience 11-0402-82

Product Code. 15208079

Description

Description: The HM40-3 monoclonal antibody reacts with mouse and rat CD40, a 45-50kDa type I transmembrane glycoprotein. CD40 is a member of the TNFR family and is expressed by mouse B lymphocytes, follicular dendritic cells, thymic epithelium, and a subset of peripheral T cells. CD40 regulates B cell development/maturation by inducing Ig isotype switching and in combination with other signals such as IL-4, protects B cells from surface Ig-induced apoptosis and promotes proliferation. Interaction of CD40 with CD154 (gp39), its ligand on T cells, is important in T-B cell crosstalk and plays a role in costimulation and immune regulation.Applications Reported: The HM40-3 antibody has been reported for use in flow cytometric analysis.Applications Tested: The HM40-3 antibody has been tested by flow cytometric analysis of mouse splenocytes and can be used at less than or equal to 1 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Excitation: 488 nm; Emission: 520 nm; Laser: Blue Laser.Filtration: 0.2μm post-manufacturing filtered.

Specifications

• Antigen: CD40
• Classification: Monoclonal
• Concentration: 0.5mg/mL
• Formulation: PBS with 0.09% sodium azide; pH 7.2
• Gene Accession No.: P27512
• Gene Alias: Bp50, CDW40, MGC9013, TNFRSF5, p50
• Host Species: Armenian Hamster
• Purification Method: Affinity chromatography
• Regulatory Status: RUO
• Type: Antibody
• Monoclonal or Polyclonal: Monoclonal
• Target Species: MurineþRat

• Applications: Flow Cytometry
• Clone: HM40-3
• Conjugate: FITC
• Gene: Cd40
• Format: Conjugated
• Gene Symbols: Cd40
• Isotype: IgM, kappa
• Quantity: 100μg
• Storage Requirements: Store at 2-8°C. Do not freeze. Light-sensitive material.
• Primary or Secondary: Primary
• Gene ID (Entrez): 21939

For Research Use Only.