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CD4 Rat anti-Canine, Super Bright 436, Clone: YKIX302.9, eBioscience™

Rat Monoclonal Antibody

Brand:  Invitrogen 62-5040-41

Additional Details : Weight : 0.01000kg

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Product Code. 15943424

  • £133.00 / Pack of 25

Estimated Shipment
21-04-2021

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Description

Description

Description: The YKIX302.9 monoclonal antibody reacts with canine CD4, a cell surface receptor expressed on a majority of thymocytes, a subpopulation of mature T cells and dendritic cells. In dogs, CD4 is also highly expressed on neutrophils. CD4 binds to MHC class II on the surface of antigen presenting cells and plays an important role in T cell development and homeostasis. This YKIX302.9 monoclonal antibody has been reported to block MHC II-restricted T cell responses in vivo and in vitro. Applications Reported: This YKIX302.9 antibody has been reported for use in flow cytometric analysis. Applications Tested: This YKIX302.9 antibody has been pre-diluted and tested by flow cytometric analysis of normal canine peripheral blood cells. This may be used at 5 μL (0.06 μg) per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. Super Bright 436 can be excited with the violet laser line (405 nm) and emits at 436 nm. We recommend using a 450/50 bandpass filter, or equivalent. Please make sure that your instrument is capable of detecting this fluorochrome. When using two or more Super Bright dye-conjugated antibodies in a staining panel, it is recommended to use Super Bright Staining Buffer (Product No. SB-4400) to minimize any non-specific polymer interactions.

Please refer to the datasheet for Super Bright Staining Buffer for more information. Excitation: 405 nm; Emission: 436 nm; Laser: Violet Laser Super Bright Polymer Dyes are sold under license from Becton, Dickinson and Company. The CD4 antigen is involved in the recognition of MHC class II molecules and is a co-receptor for HIV. CD4 is primarily expressed in a subset of T-lymphocytes, also referred to as T helper cells, but may also be expressed by other cells in the immune system, such as monocytes, macrophages, and dendritic cells. At the tissue level, CD4 expression may be detected in thymus, lymph nodes, tonsils, and spleen, and also in specific regions of the brain, gut, and other non-lymphoid tissues. CD4 functions to initiate or augment the early phase of T-cell activation through its association with the T-cell receptor complex and protein tyrosine kinase, Lck. It may also function as an important mediator of direct neuronal damage in infectious and immune-mediated diseases of the central nervous system. Multiple alternatively spliced transcripts have been identified in this gene [RefSeq, July 2017].
Specifications

Specifications

CD4
Monoclonal
5 μL/Test
PBS with BSA and 0.09% sodium azide; pH 7.2
P33705
CD4 antigen p55, CD4 receptor, T-cell surface antigen T4/Leu-3, T-cell surface glycoprotein CD4
Rat
Affinity chromatography
RUO
Antibody
Monoclonal
Canine
Flow Cytometry
YKIX302.9
Super Bright 436
CD4
Liquid
CD4
IgG2a, kappa
25 Tests
4° C, store in dark, DO NOT FREEZE!
Primary
403931
Videos
SDS
Documents