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CD4, PE-Cyanine5, clone: RM4-5, eBioscience™

Rat Monoclonal Antibody

Brand:  Affymetrix eBioscience 15-0042-81

Code : Z2

Additional Details : Weight : 0.25000kg

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Product Code. 15300170

  • £87.32 / 50µg


Estimated Shipment date
from Supplier 23-07-2018
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Description

Description

Description: The RM4-5 monoclonal antibody reacts with the mouse CD4 molecule, a 55kDa cell surface receptor expressed by a majority of thymocytes, subpopulation of mature T cells and dendritic cells. CD4 binds to MHC class II on the surface of antigen presenting cells and plays an important role both in T cell development and in optimal functioning of mature T cells. In T cells, CD4 associates with protein tyrosine kinase p56lck through its cytoplasmic tail. Binding of RM4-5 is blocked by GK1.5.Applications Reported: The RM4-5 antibody has been reported for use in flow cytometric analysis.Applications Tested: The RM4-5 antibody has been tested by flow cytometric analysis of mouse thymocytes and splenocytes. This can be used at less than or equal to 0.125 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Light sensitivity: This tandem dye is sensitive photo-induced oxidation. Please protect this vial and stained samples from light. Fixation: Samples can be stored in IC Fixation Buffer (cat. 00-8222) (100 μL cell sample + 100 μL IC Fixation Buffer) or 1-step Fix/Lyse Solution (cat. 00-5333) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically.Excitation: 488-561 nm; Emission: 667 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.Filtration: 0.2μm post-manufacturing filtered.
Specifications

Specifications

CD4
Monoclonal
0.2mg/mL
PBS with 0.1% gelatin and 0.09% sodium azide; pH 7.2
P06332
CD4 antigen p55, CD4 receptor, T-cell surface antigen T4/Leu-3, T-cell surface glycoprotein CD4
Rat
Affinity chromatography
RUO
Antibody
Monoclonal
Murine
Flow Cytometry
RM4-5
PE-Cyanine5
Cd4
Conjugated
Cd4
IgG2a, kappa
50μg
Store at 2-8°C. Do not freeze. Light-sensitive material.
Primary
12504
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