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CD4, eFluor 660, clone: GK1.5, eBioscience™
Rat Monoclonal Antibody
Brand: Affymetrix eBioscience 50-0041-80
Code : Z2
Additional Details : Weight : 0.25000kg
Description
Description: The GK1.5 monoclonal antibody reacts with the mouse CD4 molecule, a 55kDa cell surface receptor expressed by a majority of thymocytes, subpopulation of mature T cells and dendritic cells. CD4 binds to MHC class II on the surface of antigen presenting cells and plays an important role both in T cell development and in optimal functioning of mature T cells. In T cells, CD4 associates with protein tyrosine kinase p56lck through its cytoplasmic tail. Binding of GK1.5 is blocked by RM4-5.Applications Reported: This GK1.5 antibody has been reported for use in flow cytometric analysis.Applications Tested: This GK1.5 antibody has been tested by flow cytometric analysis of mouse splenocytes. This can be used at less than or equal to 0.125 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.eFluor® 660 is a replacement for Alexa Fluor® 647. eFluor® 660 emits at 659 nm and is excited with the red laser (633 nm). Please make sure that your instrument is capable of detecting this fluorochome.Excitation: 633-647 nm; Emission: 668 nm; Laser: Red Laser.Filtration: 0.2μm post-manufacturing filtered.Specifications
CD4 | |
Monoclonal | |
0.2mg/mL | |
PBS with 0.1% gelatin and 0.09% sodium azide; pH 7.2 | |
P06332 | |
CD4 antigen p55, CD4 receptor, T-cell surface antigen T4/Leu-3, T-cell surface glycoprotein CD4 | |
Rat | |
Affinity chromatography | |
RUO | |
Antibody | |
Monoclonal | |
Murine |
Flow Cytometry | |
GK1.5 | |
eFluor 660 | |
Cd4 | |
Conjugated | |
Cd4 | |
IgG2b, kappa | |
25μg | |
Store at 2-8°C. Do not freeze. Light-sensitive material. | |
Primary | |
12504 |
For Research Use Only.