Learn More
CD4 Monoclonal Antibody (RM4-5), eFluor™ 615, eBioscience™, Invitrogen™
Rat Monoclonal Antibody
Brand: Affymetrix eBioscience 42-0042-80
Code : Z2
Additional Details : Weight : 0.25000kg
Description
Description: The RM4-5 monoclonal antibody reacts with the mouse CD4 molecule, a 55 kDa cell surface receptor expressed by a majority of thymocytes, subpopulation of mature T cells and dendritic cells. CD4 binds to MHC class II on the surface of antigen presenting cells and plays an important role both in T cell development and in optimal functioning of mature T cells. In T cells, CD4 associates with protein tyrosine kinase p56lck through its cytoplasmic tail. Binding of RM4-5 is blocked by GK1.5. Applications Reported: This RM4-5 antibody has been reported for use in immunocytochemical and immunohistochemical staining of frozen tissue sections (IHC-F). Applications Tested: This Rm4-5 antibody has been tested by immunohistochemistry on frozen mouse spleen (IHC-F) at less than or equal to 1 μg/mL. It is recommended that this antibody be carefully titrated for optimal performance in the assay of interest. This product has not been validated for flow cytometric analysis. Filter Recommendation: When using this eFluor™ 615 antibody conjugate, we recommend a filter that will capture the 615 emission wavelength (for example, Excitation 560/55, 585LP, Emission 645/75). A standard Alexa Fluor™ 594 filter is acceptable. Excitation: 595 nm; Emission: 615 nm. Filtration: 0.2 μm post-manufacturing filtered.
CD4 Monoclonal antibody specifically detects CD4 in Mouse samples. It is validated for Immunocytochemistry, Immunohistochemistry (Frozen).Specifications
CD4 | |
Monoclonal | |
0.2 mg/mL | |
PBS with 0.09% sodium azide; pH 7.2 | |
P06332 | |
CD4 | |
Affinity chromatography | |
RUO | |
12504 | |
4° C, store in dark, DO NOT FREEZE! | |
Liquid |
Immunocytochemistry, Immunohistochemistry (Frozen) | |
RM4-5 | |
eFluor 615 | |
CD4 | |
Activation B7-1 antigen; B7; B7.1; B7-1; BB1; B-lymphocyte activation antigen B7; CD28LG; CD28LG1; CD4; CD4 antigen; CD4 antigen (p55); CD4 antigen p55; Cd4 molecule; CD4 precursor; CD4 receptor; CD4, allele 1; cd4a; CD4mut; CD80; CD80 antigen (CD28 antigen ligand 1, B7-1 antigen); CD80 molecule; cell surface glycoprotein CD4; costimulatory factor CD80; costimulatory molecule variant IgV-CD80; CTLA-4 counter-receptor B7.1; fCD4; L3T4; LAB7; Leu-3; Ly-4; lymphocyte antigen CD4; lymphocyte antigen CD4 precursor; membrane protein; p55; T-cell differentiation antigen L3T4; T-cell surface antigen T4/Leu-3; T-cell surface glycoprotein CD4; T-cell surface glycoprotein CD4 precursor (T-cell surface antigen T4/Leu-3) (T-cell differentiation antigen L3T4); T-lymphocyte activation antigen CD80; W3/25; W3/25 antigen | |
Rat | |
25 μg | |
Primary | |
Mouse | |
Antibody | |
IgG2a κ |
For Research Use Only.