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CD4, eFluor 615, clone: RM4-5, eBioscience™

Rat Monoclonal Antibody

Brand:  Affymetrix eBioscience 42-0042-80

Code : Z2

Additional Details : Weight : 0.25000kg

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Product Code. 15301090

  • £77.04 / 25µg


Estimated Shipment
29-06-2018
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Description

Description

Description: The RM4-5 monoclonal antibody reacts with the mouse CD4 molecule, a 55kDa cell surface receptor expressed by a majority of thymocytes, subpopulation of mature T cells and dendritic cells. CD4 binds to MHC class II on the surface of antigen presenting cells and plays an important role both in T cell development and in optimal functioning of mature T cells. In T cells, CD4 associates with protein tyrosine kinase p56lck through its cytoplasmic tail. Binding of RM4-5 is blocked by GK1.5.Applications Reported: This RM4-5 antibody has been reported for use in immunocytochemical and immunohistochemical staining of frozen tissue sections (IHC-F).Applications Tested: This Rm4-5 antibody has been tested by immunohistochemistry on frozen mouse spleen (IHC-F) at less than or equal to 1 μg/mL. It is recommended that this antibody be carefully titrated for optimal performance in the assay of interest. This product has not been validated for flow cytometric analysis.Filter Recommendation: When using this eFluor® 615 antibody conjugate, we recommend a filter that will capture the 615 emission wavelength (for example, Excitation 560/55, 585LP, Emission 645/75). A standard Alexa Fluor® 594 filter is acceptable.Excitation: 595 nm; Emission: 615 nm.Filtration: 0.2μm post-manufacturing filtered.
Specifications

Specifications

CD4
Monoclonal
0.2mg/mL
PBS with 0.1% gelatin and 0.09% sodium azide; pH 7.2
P06332
CD4 antigen p55, CD4 receptor, T-cell surface antigen T4/Leu-3, T-cell surface glycoprotein CD4
Rat
Affinity chromatography
RUO
Antibody
Monoclonal
Murine
Immunohistochemistry (Frozen)
RM4-5
eFluor 615
Cd4
Conjugated
Cd4
IgG2a, kappa
25μg
Store at 2-8°C. Do not freeze. Light-sensitive material.
Primary
12504
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