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CD4, clone: RM4-5, eBioscience™

Rat Monoclonal Antibody

Brand:  Affymetrix eBioscience 14-0042-82

Code : Z2

Additional Details : Weight : 0.01000kg

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Product Code. 15206757

  • £54.42 / 100µg


EU Stock 3
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Disclaimers:

For Research Use Only.


Description

Description

Description: The RM4-5 monoclonal antibody reacts with the mouse CD4 molecule, a 55kDa cell surface receptor expressed by a majority of thymocytes, subpopulation of mature T cells and dendritic cells. CD4 binds to MHC class II on the surface of antigen presenting cells and plays an important role both in T cell development and in optimal functioning of mature T cells. In T cells, CD4 associates with protein tyrosine kinase p56lck through its cytoplasmic tail. Binding of RM4-5 is blocked by GK1.5.Applications Reported: The RM4-5 antibody has been reported for use in flow cytometric analysis, and immunohistochemical staining. It has also been reported in isolation and depletion of CD4+ cells and blocking of ligand binding. (Please use Functional Grade purified RM4-5, cat. 16-0042, in functional assays.).Applications Tested: The RM4-5 antibody has been tested by flow cytometric analysis of mouse thymocytes and splenocytes. This can be used at less than or equal to 0.5 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Purity: Greater than 90%, as determined by SDS-PAGE.Aggregation: Less than 10%, as determined by HPLC.Filtration: 0.2μm post-manufacturing filtered.
Specifications

Specifications

CD4
RM4-5
Unconjugated
Purified
Rat
100μg
Store at 2-8°C.
Monoclonal
Immunohistochemistry (Frozen)
0.5mg/mL
aqueous buffer, 0.09% sodium azide, may contain carrier protein/stabilizer
L3T4, Ly-4
IgG2a
RUO
Primary
Murine
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