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CD4, PerCP-eFluor™ 710, clone: OX35, eBioscience™

Mouse Monoclonal Antibody

Brand:  Affymetrix eBioscience 46-0040-82

Code : Z2

Additional Details : Weight : 0.25000kg

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Product Code. 15351220

  • £174.23 / 100µg


Estimated Shipment
27-02-2018
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Disclaimers:

For Research Use Only.


Description

Description

Description: The OX35 monoclonal antibody reacts with rat CD4. CD4 is a co-receptor which participates in signaling through the T cell receptor by making contacts with MHC class II expressed on antigen-presenting cells (APC). CD4 is expressed in the thymus by CD4+CD8+ double-positive and CD4+ single-positive thymocytes, and by CD4+ helper T cells in the periphery. CD4 is a cell-surface membrane glycoprotein which contains four extracellular immunoglobulin-like domains.Applications Reported: This OX35 antibody has been reported for use in flow cytometric analysis.Applications Tested: This OX35 antibody has been tested by flow cytometric analysis of rat splenocytes. This can be used at less than or equal to 0.25 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.PerCP-eFluor® 710 emits at 710 nm and is excited with the blue laser (488 nm); it can be used in place of PerCP-Cyanine5.5. We recommend using a 710/50 bandpass filter, however, the 695/40 bandpass filter is an acceptable alternative. Please make sure that your instrument is capable of detecting this fluorochrome.Fixation: Samples can be stored in IC Fixation Buffer (cat. 00-8222) (100 μL cell sample + 100 μL IC Fixation Buffer) or 1-step Fix/Lyse Solution (cat. 00-5333) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically.Excitation: 488 nm; Emission: 710 nm; Laser: Blue Laser.Filtration: 0.2μm post-manufacturing filtered.
Specifications

Specifications

CD4
OX35
PerCP-eFluor 710
Conjugated
Murine
100μg
Store at 2-8°C. Do not freeze. Light-sensitive material.
Monoclonal
Flow Cytometry
0.2mg/mL
aqueous buffer, 0.09% sodium azide, may contain carrier protein/stabilizer
W3/25
IgG2a
RUO
Primary
Rat
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