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CD4, eVolve 605, clone: SK3 (SK-3), eBioscience™

Mouse Monoclonal Antibody

Brand:  Affymetrix eBioscience 83-0047-42

Code : NEW

Additional Details : Weight : 0.23750kg

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Product Code. 15570737

This item has been discontinued and is no longer available. View the product for possible alternatives or contact our Product Support team on 01509 555888 for assistance


Description

Description

Description: The SK3 monoclonal antibody reacts with human CD4, a 59-kDa cell surface receptor expressed by a majority of thymocytes, a subpopulation of mature T helper cells, and at low levels on monocytes. CD4 is a receptor for the human immunodeficiency virus (HIV). SK3 blocks HIV binding and mixed lymphocyte reaction. The SK3 and RPA-T4 monoclonal antibodies do not cross-block binding, suggesting recognition of distinct epitopes.Applications Reported: This SK3 (SK-3) antibody has been reported for use in flow cytometric analysis.Applications Tested: This SK3 (SK-3) antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at 5 μL per test. A test is defined as the amount of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. For best results when using antibodies conjugated to eVolve™, we recommend a quick spin of the vial before use and avoid touching the bottom of the vial with the pipet tip.Laser/Filter Recommendation: eVolve™ 605 can be optimally excited with laser lines from 325-405 nm, and emits near 610 nm. We recommend using the violet (405 nm) laser line for excitation and a 595LP with a 605/40 or 610/20 bandpass filter, or equivalent, for detection.Fixation Recommendation: Antibodies conjugated to eVolve™ are compatible with eBioscience's fixation and permeabilization buffers.Excitation: 350-590 nm; Emission: 605 nm; Laser: Ultraviolet Laser, Violet LaserStorage and handling: For best results when using antibodies conjugated to eVolve™, we recommend a quick spin of the vial before use and avoid touching the bottom of the vial with the pipet tip.
Specifications

Specifications

CD4
Monoclonal
5μl/Test
PBS with 0.2% BSA and 0.09% sodium azide; pH 7.2
P01730
CD4 antigen p55, CD4 receptor, T-cell surface antigen T4/Leu-3, T-cell surface glycoprotein CD4
Murine
Affinity chromatography
RUO
Antibody
Monoclonal
Human
Flow Cytometry
SK3 (SK-3)
eVolve 605
CD4
Conjugated
CD4
IgG1, kappa
100 Tests
Store at 2-8°C. Do not freeze. Light-sensitive material. This product should be used within 6 months from the date of receipt.
Primary
920
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