CD4, eVolve 605, clone: SK3 (SK-3), eBioscience™

Mouse Monoclonal Antibody

Brand:  Affymetrix eBioscience 83-0047-41

Product Code. 15560737

Description

Description: The SK3 monoclonal antibody reacts with human CD4, a 59-kDa cell surface receptor expressed by a majority of thymocytes, a subpopulation of mature T helper cells, and at low levels on monocytes. CD4 is a receptor for the human immunodeficiency virus (HIV). SK3 blocks HIV binding and mixed lymphocyte reaction. The SK3 and RPA-T4 monoclonal antibodies do not cross-block binding, suggesting recognition of distinct epitopes.Applications Reported: This SK3 (SK-3) antibody has been reported for use in flow cytometric analysis.Applications Tested: This SK3 (SK-3) antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at 5 μL per test. A test is defined as the amount of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. For best results when using antibodies conjugated to eVolve™, we recommend a quick spin of the vial before use and avoid touching the bottom of the vial with the pipet tip.Laser/Filter Recommendation: eVolve™ 605 can be optimally excited with laser lines from 325-405 nm, and emits near 610 nm. We recommend using the violet (405 nm) laser line for excitation and a 595LP with a 605/40 or 610/20 bandpass filter, or equivalent, for detection.Fixation Recommendation: Antibodies conjugated to eVolve™ are compatible with eBioscience's fixation and permeabilization buffers.Excitation: 350-590 nm; Emission: 605 nm; Laser: Ultraviolet Laser, Violet LaserStorage and handling: For best results when using antibodies conjugated to eVolve™, we recommend a quick spin of the vial before use and avoid touching the bottom of the vial with the pipet tip.

Specifications

• Antigen: CD4
• Classification: Monoclonal
• Concentration: 5μl/Test
• Formulation: PBS with 0.2% BSA and 0.09% sodium azide; pH 7.2
• Gene Accession No.: P01730
• Gene Alias: CD4 antigen p55, CD4 receptor, T-cell surface antigen T4/Leu-3, T-cell surface glycoprotein CD4
• Host Species: Murine
• Purification Method: Affinity chromatography
• Regulatory Status: RUO
• Type: Antibody
• Monoclonal or Polyclonal: Monoclonal
• Target Species: Human

• Applications: Flow Cytometry
• Clone: SK3 (SK-3)
• Conjugate: eVolve 605
• Gene: CD4
• Format: Conjugated
• Gene Symbols: CD4
• Isotype: IgG1, kappa
• Quantity: 25 Tests
• Storage Requirements: Store at 2-8°C. Do not freeze. Light-sensitive material. This product should be used within 6 months from the date of receipt.
• Primary or Secondary: Primary
• Gene ID (Entrez): 920

For Research Use Only.