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CD4, eVolve 605, clone: SK3 (SK-3), eBioscience™
Mouse Monoclonal Antibody
Brand: Affymetrix eBioscience 83-0047-41
Code : NEW
Additional Details : Weight : 0.23750kg
Description
Description: The SK3 monoclonal antibody reacts with human CD4, a 59-kDa cell surface receptor expressed by a majority of thymocytes, a subpopulation of mature T helper cells, and at low levels on monocytes. CD4 is a receptor for the human immunodeficiency virus (HIV). SK3 blocks HIV binding and mixed lymphocyte reaction. The SK3 and RPA-T4 monoclonal antibodies do not cross-block binding, suggesting recognition of distinct epitopes.Applications Reported: This SK3 (SK-3) antibody has been reported for use in flow cytometric analysis.Applications Tested: This SK3 (SK-3) antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at 5 μL per test. A test is defined as the amount of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. For best results when using antibodies conjugated to eVolve™, we recommend a quick spin of the vial before use and avoid touching the bottom of the vial with the pipet tip.Laser/Filter Recommendation: eVolve™ 605 can be optimally excited with laser lines from 325-405 nm, and emits near 610 nm. We recommend using the violet (405 nm) laser line for excitation and a 595LP with a 605/40 or 610/20 bandpass filter, or equivalent, for detection.Fixation Recommendation: Antibodies conjugated to eVolve™ are compatible with eBioscience's fixation and permeabilization buffers.Excitation: 350-590 nm; Emission: 605 nm; Laser: Ultraviolet Laser, Violet LaserStorage and handling: For best results when using antibodies conjugated to eVolve™, we recommend a quick spin of the vial before use and avoid touching the bottom of the vial with the pipet tip.Specifications
CD4 | |
Monoclonal | |
5μl/Test | |
PBS with 0.2% BSA and 0.09% sodium azide; pH 7.2 | |
P01730 | |
CD4 antigen p55, CD4 receptor, T-cell surface antigen T4/Leu-3, T-cell surface glycoprotein CD4 | |
Murine | |
Affinity chromatography | |
RUO | |
Antibody | |
Monoclonal | |
Human |
Flow Cytometry | |
SK3 (SK-3) | |
eVolve 605 | |
CD4 | |
Conjugated | |
CD4 | |
IgG1, kappa | |
25 Tests | |
Store at 2-8°C. Do not freeze. Light-sensitive material. This product should be used within 6 months from the date of receipt. | |
Primary | |
920 |
For Research Use Only.