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CD4, eFluor 450, clone: RPA-T4, eBioscience™

Mouse Monoclonal Antibody

Brand:  Affymetrix eBioscience 48-0049-41

Code : NEW

Additional Details : Weight : 0.23750kg

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Product Code. 15509626

  • £67.59 / 25 tests


Estimated Shipment date
from Supplier 26-03-2018
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Disclaimers:

For Research Use Only.


Description

Description

Description: The RPA-T4 monoclonal antibody reacts with human CD4, a 59kDa cell surface receptor expressed by a majority of thymocytes, subpopulation of mature T cells (T-helper cells) and in low levels on monocytes. CD4 is a receptor for the human immunodeficiency virus (HIV). RPA-T4 blocks HIV binding and mixed lymphocyte reaction. The RPA-T4 antibody recognizes a different epitope than the OKT4 monoclonal antibody, and these antibodies do not cross-block binding to each other's respective epitopes.Applications Reported: This RPA-T4 antibody has been reported for use in flow cytometric analysis.Applications Tested: This RPA-T4 antibody has been pre-titrated and tested by flow cytometric analysis of human peripheral mononuclear cells. This can be used at 5 μL (0.25 μg) per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. eFluor® 450 is an alternative to Pacific Blue®. eFluor® 450 emits at 445 nm and is excited with the Violet laser (405 nm). Please make sure that your instrument is capable of detecting this fluorochome.Excitation: 405 nm; Emission: 445 nm; Laser: Violet Laser.Filtration: 0.2μm post-manufacturing filtered.
Specifications

Specifications

CD4
RPA-T4
eFluor 450
Conjugated
Murine
25 Tests
Store at 2-8°C. Do not freeze. Light-sensitive material.
Monoclonal
Flow Cytometry
5μL (0.25μg)/test
aqueous buffer, 0.09% sodium azide, may contain carrier protein/stabilizer
Leu-3, T4
IgG1
RUO
Primary
Human
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